饲喂益生菌Lactobacillus casei Zhang对高脂日粮大鼠肝脏脂质代谢的作用

【目的】研究不同剂量益生菌L. casei Zhang（2.0×1010 CFU/d、2.0×109 CFU/d和2.0×108 CFU/d）及其发酵乳饮料（2.0×108 CFU/mL/d）对高胆固醇血症大鼠肝脏脂质的治疗作用。【方法】采用高脂饲料诱导高脂血症，采用相应试剂盒测定肝脏总胆固醇水平（TC）和甘油三酯（TG）水平、粪便总胆汁酸水平与胆固醇水平以及血清载脂蛋白ApoAⅠ与ApoB水平。【结果】益生菌各剂量组和发酵饮料组与高脂模型组相比，肝脏总胆固醇水平（TC）和甘油三酯（TG）水平均显著降低(P＜0.05 或P＜0.01)。各试验组均可显著增加高血脂大鼠粪便总胆汁酸水平和血清载脂蛋白ApoAⅠ水平以及显著降低血清载脂蛋白ApoB水平(P＜0.01)，但对粪便胆固醇水平影响不明显(P＞0.05)。【结论】益生菌L. casei Zhang主要通过增加总胆汁酸的排出和调节载脂蛋白水平来改善高血脂大鼠肝脏脂质水平。

Effect of Lactobacillus casei Zhang Administration on Liver Lipids Metabolism of High-Fat Diet Induced Hypercholesterolemia Rats

【Objective】 The therapeutic effect of different doses of L.casei Zhang(2.0×1010 CFU/d,2.0×109 CFU/d and 2.0×108 CFU/d) and its fermented milk beverage(2.0×108 CFU/mL/d) on liver lipids of hypercholesterolemia rats was investigated.【Method】Hypercholesterolemia rats were induced by high fat feeding.Liver TC and TG levels,fecal total bile acid and cholesterol levels as well as serum ApoAⅠand ApoB levels were dertermined by commercial kits.【Result】 The results showed that liver TC and TG levels were significantly decreased in all probiotic treatment groups compared to hypercholesterolemia model group(P<0.05 or P<0.01).In addition,fecal total bile acid levels and serum ApoAⅠlevels were significantly promoted and serum ApoB levels significantly reduced in all probiotic treatment groups compared to model group,while fecal cholesterol levels were changed with no significance(P>0.05).【Conclusion】 It is suggested that enhancing elimination of bile acids and regulating apolipoprotein levels could be mainly contributed to liver lipids ameliorated by probiotic L.casei Zhang in hypercholesterolemia rats.