| 人参叶片愈伤组织诱导数学模型分析 |
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| 引用本文： | 任宣百,陆志民,李金清,李伟. 人参叶片愈伤组织诱导数学模型分析[J]. 吉林林业科技, 2016, 0(2). DOI: 10.16115/j.cnki.issn.1005-7129.2016.02.006 |
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| 作者姓名： | 任宣百 陆志民 李金清 李伟 |
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| 作者单位： | 1. 吉林省林业科学研究院,吉林长春,130033;2. 集安市林业局,吉林通化,134200 |
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| 基金项目： | 国家林业局“948”项目(2004－4－20) |
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| 摘 要： | 取人参叶片作为外植体诱导愈伤组织,利用二元二次通用旋转组合设计数学模型筛选植物激素浓度组合,结果表明:2,4-D与KT对人参叶片外植体诱导愈伤组织方程为:Y=0.653 30+0.229 63X1+0.140 85X2-0.130 83X12-0.197 48X22+0.100 00X1X2;叶片诱导愈伤组织最佳培养基为MS附加7 mg·L-12,4-D和0.9 mg·L-1KT,诱导率最高可达80%以上。
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| 关 键 词： | 人参叶片 愈伤组织 诱导率 数学模型 通用旋转组合设计 |
The mathematical model analysis of Panax ginseng lamina callus induction |
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| Abstract: | The plant hormone concentration combinations was selected with the mathematical model designed by dyadic and square general rotary combination by obtaining the Panax ginseng lamina as explant to induce the callus.The result showed that the equation of 2,4 -D and KT of Panax ginseng lamina callus induction was Y =0.653 30 +0.229 63X1 +0.140 85X2 -0.130 83X1 2 -0.197 48X2 2 +0.100 00X1 X2 .The optimum medium of lamina induction callus was MS add-ed to 7 mg· L-1 2,4-D and 0.9 mg· L-1 KT.The maximum inductivity was more than 80%. |
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| Keywords: | Panax ginseng lamina callus inductivity mathematical model general rotary combination design |
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