| 桦褐孔菌多酚的提取条件优化和抗氧化能力研究 |
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| 引用本文： | 潘林,李亚楠,张志,朱蕴兰.桦褐孔菌多酚的提取条件优化和抗氧化能力研究[J].农业工程,2022,12(5):53-57. |
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| 作者姓名： | 潘林 李亚楠 张志 朱蕴兰 |
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| 作者单位： | 1.齐齐哈尔大学生命与农林学院，抗性基因工程与寒地生物多样性保护重点实验室，黑龙江 齐齐哈尔 161005 |
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| 基金项目： | 国家自然基金面上项目(31670375),徐州市推动科技创新专项重点研发计划(KC17057)。 |
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| 摘 要： | 以桦褐孔菌菌核为试验材料，利用超声?微波协同萃取法提取了桦褐孔菌样品中的多酚，采用单因素和多因素试验优化了桦褐孔菌多酚的提取条件，测定了多酚的抗氧化能力。试验结果显示，超声?微波协同萃取法提取桦褐孔菌多酚的优化条件是乙醇体积分数50%、料液比1∶60、微波功率400 W、超声波功率350 W、提取时间240 s，此条件下的多酚提取率为2.943%。该方法提取的多酚具有良好的抗氧化能力，对DPPH自由基清除率的IC50值为9.03 μg/mL，对羟自由基清除率的IC50值为2.03 mg/mL。该方法是一种实用、有效、快速的提取桦褐孔菌多酚的方法。
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| 关 键 词： | 桦褐孔菌 多酚 超声?微波协同萃取法 抗氧化能力 |
| 收稿时间： | 2021/11/29 0:00:00 |
| 修稿时间： | 2022/1/29 0:00:00 |
Optimization of extraction conditions and antioxidant capacity of polyphenols from Inonotus obliquus |
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| PAN Lin,LI Ya-nan,Zhnag Zhi and ZHU Yun-lan.Optimization of extraction conditions and antioxidant capacity of polyphenols from Inonotus obliquus[J].Agricultural Engineering,2022,12(5):53-57. |
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| Authors: | PAN Lin LI Ya-nan Zhnag Zhi and ZHU Yun-lan |
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| Institution: | 1.Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas，College of Life Sciences and Agriculture and Forestry，Qiqihar University，Qiqihar Heilongjiang 161005，China2.College of Food and Biology Engineering，Xuzhou University of Technology，Jiangsu Research Center of Food and Biology Process Engineering Technology，Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe，Xuzhou Jiangsu 221018，China |
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| Abstract: | Polyphenols were extracted by ultrasonic-microwave co-extraction with sclerotia of Inonotus obliquus．Extraction conditions of polyphenols from Inonotus obliquus were optimized by single factor and multi-factor experiments, and antioxidant capacity of polyphenols was determined．Results showed that optimal extraction conditions were as follows: ethanol volume fraction of 50%, solid-liquid ratio of 1∶60, microwave power of 400 W, ultrasonic power of 350 W and extraction time of 240 s．Under these conditions, extraction rate of polyphenols was 2.943%．Polyphenols extracted by this method had good antioxidant capacity．The IC50 of DPPH free radical scavenging was 9.03 μg/mL, and the IC50 of hydroxyl free radical scavenging was 2.03 mg/mL．This method is a practical, effective and rapid method for the extraction of polyphenols from Inonotus obliquus． |
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| Keywords: | Inonotus obliquus polyphenols antioxidant |
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