| 薰衣草茎段再生体系的建立 |
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| 引用本文： | 何家涛,王会,董珍文,赵劲松.薰衣草茎段再生体系的建立[J].江西农业学报,2006,18(4):91-93. |
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| 作者姓名： | 何家涛 王会 董珍文 赵劲松 |
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| 作者单位： | 襄樊职业技术学院,湖北,襄樊,441021 |
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| 基金项目： | 湖北省襄樊市科技局科研项目 |
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| 摘 要： | 以薰衣草带芽茎段为外植体,探讨不同激素种类与水平等因素对其愈伤诱导、不定芽分化、增殖与不定根形成的影响,并筛选了薰衣草试管苗过渡移栽的适宜基质等。试验结果表明:不定芽启动培养以MS+BA1.0~2.0mg/L+蔗糖30g/L+琼脂6.5g/L为宜;继代增殖以MS+BA1.0~2.0mg/L+IBA0.25mg/L+GA1.0mg/L+蔗糖30g/L+琼脂6.5g/L为宜;不定根分化以White+IBA0.4mg/L+蔗糖20g/L+琼脂6.5g/L或White+NAA0.8mg/L+蔗糖20g/L+琼脂6.5 g/L为宜。
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| 关 键 词： | 薰衣草 茎段 离体培养 植株再生 外植体 |
| 文章编号： | 1001-8581（2006）04-0091-03 |
| 收稿时间： | 2006-03-27 |
| 修稿时间： | 2006年3月27日 |
Establishment of Regeneration System of Stem of Lavandula angustifolia |
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| HE Jia-tao,WANG Hui,DONG Zhen-wen,ZHAO Jin-song.Establishment of Regeneration System of Stem of Lavandula angustifolia[J].Acta Agriculturae Jiangxi,2006,18(4):91-93. |
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| Authors: | HE Jia-tao WANG Hui DONG Zhen-wen ZHAO Jin-song |
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| Institution: | Department of Biological Engineering, Xiangfan Vocational and Technology College, Xiangfan 441021, China |
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| Abstract: | The study is made to probe into the effect of various hormones on the explant based on the stem of Lavandula angustifolia about induced callus,adventitious buds differentiation,multiplication and adventitious roots forming.The results show that MS BA1.0~2.0mg/L cane sugar 30g/L agar 6.5g/L will be suitable for the fostering starting of adventitious buds,MS BA1.0~2.0mg/L IBA0.25mg/L GA1.0mg/L sucrose 30g/L agar 6.5g/L has better effect on multiplication,White IBA0.4mg/L sucrose 20g/L agar 6.5g/L or White NAA0.8mg/L cane sugar 20g/L agar 6.5g/L has a better effect on adventitious buds differentiation,the optimum transplanting substrate is peat:vermiculite(1:1).The survival rate of test-tube seedlings reaches 96.8%. |
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| Keywords: | Lavandula angustifolia Stem In vitro culture Plant regeneration Explant |
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