Extraction, radioiodination, and in vivo catabolism of equine fibrinogen |
| |
Authors: | C P Coyne W J Hornof A B Kelly T R O'Brien S J DeNardo |
| |
Abstract: | Equine fibrinogen was isolated and aliquots were stored frozen at -70 C before radiolabeling with 125I (half-life = 60.2 days; gamma = 35 keV, using monochloroiodine reagent. Radioiodination efficiencies were 49% to 53%, resulting in a labeled product with 98% protein-bound activity and 91% clottable radioactivity. In 6 equine in vivo investigations, plasma half-lives of 125I-labeled fibrinogen were from 4.1 to 5.2 days, corresponding to a mean daily plasma elimination rate of approximately 15%. |
| |
Keywords: | |
|
|