Efficacy of Vaccination Against Streptococcus iniae During Artificial Spawning of the Red-Tail Black Shark (Epalzeorhynchos bicolor,fam. Cyprinidae) |
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| Authors: | Riccardo Russo Roy P. E. Yanong |
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| Affiliation: | 1. Tropical Aquaculture Laboratory, Program in Fisheries and Acquatic Sciences, SFRC , Institute of Food and Agricultural Sciences, University of Florida , Ruskin, Florida, USA ric.russo@ars.usda.gov ri.russo@hotmail.com;3. Tropical Aquaculture Laboratory, Program in Fisheries and Acquatic Sciences, SFRC , Institute of Food and Agricultural Sciences, University of Florida , Ruskin, Florida, USA |
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| Abstract: | Artificial spawning may precipitate high mortalities of red-tail black shark (Epalzeorhynchos bicolor) broodstock from Streptococcus iniae infection. Two vaccine formulations (aluminum or oil adjuvant) were evaluated for effectiveness. Fish (mean weight 21.2 ± 5.7 g) were harvested, acclimatized, and vaccinated by intracoelomic injection. After 21 days, fish were moved to a biosecure laboratory and, one day later, simultaneously spawned and challenged by bath or by intracoelomic injection with S. iniae. Both formulations increased relative% survival (RPS) in unspawned (RPS [formulation]: 80 [aluminum]; 54 [oil]) and spawned fish (87–95 [aluminum]; 73–75 [oil]), suggesting that vaccination increases survival of red-tail black shark broodstock after spawning. |
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| Keywords: | Streptococcus iniae cyprinid red-tail black shark vaccine spawning ornamental fish |
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