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水稻矮缩病毒基因组遗传多样性的初步研究
引用本文:章松柏,吴祖建,段永平,王盛,林奇英,谢联辉.水稻矮缩病毒基因组遗传多样性的初步研究[J].长江大学学报,2009(4):37-39.
作者姓名:章松柏  吴祖建  段永平  王盛  林奇英  谢联辉
作者单位:[1]长江大学农学院,湖北荆州434025 [2]福建农林大学农药生物化学教育部重点实验室,福建福州350002
基金项目:国家自然科学基金项目(39900091)
摘    要:田间调查发现,云南、福建两地的水稻矮缩病症状存在差异。生物学接种实验排除了水稻品种引起这种差异的可能性。通过10%SDS-PAGE对福建、云南两地的7个水稻矮缩病毒分离物基因组遗传多样性进行分析,结果表明基因组之间存在明显差异,主要体现在S2、S3、S11、S124个基因片段上,其中S2、S3片段之间的差异与症状差异有地域上的一致性,推测这4个基因片段中的一个或几个与病毒的致病性相关。

关 键 词:水稻矮缩病毒  遗传多样性  SDS-聚丙烯酰胺凝胶电泳

Preliminary Study on Genome Genetic Diversity of Rice Dwarf Virus
ZHANG Song-bai College of Agronomy,Yangtze University,Jingzhou,Hubei ,China,Key Laboratory of Pesticide , Biochemistry,State Education Commission,Fujian Agriculture , Forestry University,Fuzhou,Fujian ,China WANG Sheng,WU Zu-jian DUAN Yong-ping,XIE Lian-hui Key Laboratory of Pesticide , Biochemistry.Preliminary Study on Genome Genetic Diversity of Rice Dwarf Virus[J].Journal of Yangtze University,2009(4):37-39.
Authors:ZHANG Song-bai College of Agronomy  Yangtze University  Jingzhou  Hubei  China  Key Laboratory of Pesticide  Biochemistry  State Education Commission  Fujian Agriculture  Forestry University  Fuzhou  Fujian  China WANG Sheng  WU Zu-jian DUAN Yong-ping  XIE Lian-hui Key Laboratory of Pesticide  Biochemistry
Institution:ZHANG Song-bai College of Agronomy,Yangtze University,Jingzhou,Hubei 434025,China,Key Laboratory of Pesticide , Biochemistry,State Education Commission,Fujian Agriculture , Forestry University,Fuzhou,Fujian 350002,China WANG Sheng,WU Zu-jian DUAN Yong-ping,XIE Lian-hui Key Laboratory of Pesticide , Biochemistry
Abstract:The survey in fields showed that the rice disease symptom caused by RDV was different from isolates in Fujian and Yunnan.Biological inoculation experiment conformed that symptom difference was not caused by rice variety.By 10% SDS-PAGE,the genome dsRNA of seven RDV isolates from Fujian and Yunnan provinces were analyzed,and distinct varibility was showed between the genes S2,S3,S11,S12 from these isolates.Farthermore,the varibility between S2/S3 fragments showed regionally accordance with symptom difference...
Keywords:rice dwarf virus  genetic diversity  SDS-PAGE  
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