| 农杆菌介导法遗传转化巴西橡胶树转化条件的研究 |
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| 引用本文： | 洪磊,王颖,张秀娟,陈雄庭.农杆菌介导法遗传转化巴西橡胶树转化条件的研究[J].农村实用技术与信息,2009(5):38-40,63. |
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| 作者姓名： | 洪磊 王颖 张秀娟 陈雄庭 |
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| 作者单位： | [1]中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南海口571101 [2]海南大学,海南海口570228 |
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| 摘 要： | 用包含GAI基因的EHA105农杆菌，对巴西橡胶树海垦2花药愈伤组织进行侵染，对多项转化条件进行了梯度实验。结果表明选择培养30d的橡胶花药愈伤组织，农杆菌菌液浓度OD600为0．6时侵染3min，20℃共培养2d，Cef浓度为200mg／L进行抑菌培养是比较合理的转化条件。
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| 关 键 词： | 巴西橡胶 农杆菌 GAI基因 |
Study on the Conditions of the Agrobacterium -mediated Genetic Transformation for Hevea Brasiliensis |
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| Institution: | Hong Lei, Wang Ying, Zhang Xiu- juan, Chen Xiongting (1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscienee and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou,Hainan 571101 China; 2. Hainan University, Haikou 570228, China) |
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| Abstract: | The Agrobacterium containing GAI gene was used to infect the anther callus of Haiken 2 - a variety of Hevea brasiliensis to get a transgenie plant. The gradient experiments of several transformation conditions were done. The results showed that： the anther callus cultured for 30 days, 0.6 OD600 value of the Agrobacterium solution, 3min infection time, co -culture for 2 days at 20℃ and 200 mg/L Cef concentration for Agrobacterium inhibition culture were optimal transformation conditions. |
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| Keywords: | Hevea brasiliensis Agrobacterium GAI gene |
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