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| 21个兰花品种的延长RAPD(ERAPD)和PCR-RFLP分析 | |
| 引用本文： | 蹇黎,武海,张以忠,于丹凤,秦小军,朱利泉.21个兰花品种的延长RAPD(ERAPD)和PCR-RFLP分析[J].中国农学通报,2010,26(14):232-237. |
| 作者姓名： | 蹇黎 武海 张以忠 于丹凤 秦小军 朱利泉 |
| 作者单位： | 1. 西南大学农学与生物技术学院,重庆,400715;毕节学院环境与生命科学系,贵州,毕节,551700 2. 西南大学农学与生物技术学院,重庆,400715 3. 毕节学院环境与生命科学系,贵州,毕节,551700 |
| 基金项目： | 贵州省教育厅自然科学研究项目 |
| 摘 要： | 【研究目的】明确中国兰花品种间在DNA水平上的遗传多样性和亲缘关系。【方法】应用ERAPD（延长随机引物扩增）、叶绿体和线粒体基因组PCR-RFLP对21个中国兰花品种进行分子鉴定。【结果】RAPD分析中共有8个(10%)引物和88条(83.81%)多态性带纹；ERAPD分析中能获得一条2.5KbRAPD特异性片段；叶绿体基因组（cpDNA）的PCR-RFLP分析中有4个（66.67%）引物和12个(25%)引物-酶组合；线粒体基因组中（mtDNA）的PCR-RFLP分析中有2个（33.33%）引物和1个（4%）引物-酶组合能揭示材料间遗传差异。【结论】3种分子标记均能揭示品种间在DNA水平上存在遗传差异，而且遗传学分类与形态学分类基本吻合。 |
| 关 键 词： | 玉米 玉米 马唐 间作 重金属 生物富集 |
| 收稿时间： | 2010/3/22 0:00:00 |
| 修稿时间： | 4/2/2010 12:00:00 AM |
Molecular Characterization of Chinese cymbidium based on Extended RAPD (ERAPD) and PCR-RFLP Markers |
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| Jian Li,Wu Hai,Zhang Yizhong,Yu Danfeng,Qin Xiaojun,Zhu Liquan.Molecular Characterization of Chinese cymbidium based on Extended RAPD (ERAPD) and PCR-RFLP Markers[J].Chinese Agricultural Science Bulletin,2010,26(14):232-237. | |
| Authors: | Jian Li Wu Hai Zhang Yizhong Yu Danfeng Qin Xiaojun Zhu Liquan |
| Institution: | 1 College of Agronomy and Biotechnology,Southwest University,Chongqing 400715;2 Department of Environment and Life Science,BiJie College,Bijie Guizhou 551700) |
| Abstract: | In order to reveal the genetic variations and relationship of 21 Chinese Cymbidium cultivars.21 cultivars from 7 cymbidium species were analyzed by ERAPD,cpDNA and mtDNA PCR-RFLP markers.The results showed that 88 polymorphic bands were observed in 8 out of 80 random primers（10% ） in RAPD analyses,the 2.5 Kb RAPD fragment were obtained in ERAPD analysis,genetic difference were revealed in 4 primers（66.67% ） and 12 primer-enzyme combinations（25% ） by cpDNA PCR-RFLP analysis,2 primers （33.33%） and 1 primer-enzyme combinations（4%） by mtDNA PCR-RFLP analysis.The results indicated that these molecular markers can detect the genetic difference in 21 Chinese Cymbidium cultivars. |
| Keywords: | PCR-RFLP |
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