| 仙人掌超氧化物歧化酶提取与纯化工艺研究 |
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| 引用本文： | 王中凤,张敏,杨红,李奇,马正耕. 仙人掌超氧化物歧化酶提取与纯化工艺研究[J]. 中国林副特产, 2009, 0(6): 13-16 |
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| 作者姓名： | 王中凤 张敏 杨红 李奇 马正耕 |
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| 作者单位： | 合肥学院生物与环境工程系,合肥,230022 |
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| 基金项目： | 合肥学院人才引进基金 |
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| 摘 要： | 为了实现对仙人掌资源的深度开发利用,试验研究了以规模化种植的食用仙人掌为材料,提取和纯化SOD的工艺路线和相关参数。去除新鲜仙人掌表面的刺和蜡质、清洗、榨汁、粗滤、超声波处理、离心,得仙人掌粗酶液,再经热处理、硫酸铵盐析和SephadexG-100柱层析分离纯化,高分子聚合物脱水,真空冷冻干燥,获得仙人掌SOD粗酶粉,得率为13.3mg/100g.fw,酶活力为860U/mg。超声波处理能够显著降低仙人掌原浆的黏稠度,提高出汁率,相应提高从原浆里提取SOD的得率。通过正交试验,超声波功率600W、温度40℃与时间20min的组合,所得仙人掌汁液SOD活力收率最高,达215.6U/mL。硫酸铵饱和度40%和85%分别用于除杂质和沉淀SOD目标蛋白具有纯化和浓缩的双重效果。
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| 关 键 词： | 仙人掌 超氧化物歧化酶(SOD) 提取 纯化 |
Study on the Technique for Extraction and Purification of Superoxide Dismutase from Cactus |
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| Wang Zhongfeng,Zhang Ming,Yang Hong,Li Qi,Ma Zhenggeng. Study on the Technique for Extraction and Purification of Superoxide Dismutase from Cactus[J]. Forest By-product and Speciality in China, 2009, 0(6): 13-16 |
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| Authors: | Wang Zhongfeng Zhang Ming Yang Hong Li Qi Ma Zhenggeng |
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| Affiliation: | Wang Zhongfeng,Zhang Ming,Yang Hong,Li Qi,Ma Zhenggeng(Department of biological , environmental engineering,Hefei University,Hefei 230022) |
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| Abstract: | The technique for extraction and purification of superoxide dismutase from edible cactus was studied in this experiment for further making use of cactus.Prick and wax being removed,the fresh edible cactus was cleaned,pulped,filtrated,and treated by sonifer and centrifuged.The crude superoxide dismutase(SOD) liquid was obtained.Heat treatment,ammonia sulfate and Sephadex G-100 gel chromatography columns were employed for primary purification of SOD.The solution containing SOD was concentrated by polyethylene... |
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| Keywords: | Cactus Superoxide dismutase Extraction Purification |
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