| 壳聚糖固定化红树莓超氧化物歧化酶的研究 |
| |
| 引用本文： | 宣景宏,张春艳,孟宪军.壳聚糖固定化红树莓超氧化物歧化酶的研究[J].北方园艺,2007(2):31-33. |
| |
| 作者姓名： | 宣景宏 张春艳 孟宪军 |
| |
| 作者单位： | 1. 沈阳农业大学食品学院,辽宁,110161;辽宁省果蚕管理总站,沈阳,110034
2. 沈阳农业大学食品学院,辽宁,110161 |
| |
| 摘 要： | 研究了壳聚糖固定化超氧化物歧化酶的酶学性质。分别以不同方法对超氧化物歧化酶进行固定并比较其活力,对固定化方法进行相应的优化,对固定化超氧化物歧化酶进行酶学性质测定;研究结果表明以壳聚糖为载体,戊二醛交联法制备固定化超氧化物歧化酶,优化条件下制备的固定化酶,所得壳聚糖酶粉活力较高,热稳定性和酸碱稳定性较游离酶有很大的提高,且具有良好的贮存稳定性;结论是壳聚糖-戊二醛交联法可用于制备性能较优的固定化超氧化物歧化酶。
|
| 关 键 词： | 超氧化物歧化酶 壳聚糖 固定化酶 |
| 文章编号： | 1001-0009(2007)02-0031-03 |
| 收稿时间： | 11 11 2006 12:00AM |
| 修稿时间： | 2006年11月11日 |
Study on the Immobilization of Supeoxide dismutase in Red Raspberry with Chitosan |
| |
| XUAN Jing-hong,ZHANG Chun-yan,MENG Xian-jun.Study on the Immobilization of Supeoxide dismutase in Red Raspberry with Chitosan[J].Northern Horticulture,2007(2):31-33. |
| |
| Authors: | XUAN Jing-hong ZHANG Chun-yan MENG Xian-jun |
| |
| Institution: | 1. Shenyang Agriculture University Food Science College , Shenyang 110160; 2. Fruit and Silkworm Administrative General Station of Liaoning Province, shenyang 110034 |
| |
| Abstract: | Studied the enzyme properties of immobilized superoxide dismutase with chitosan. Immobilized superoxide dismutase were prepared by different process. The methods were optimized by comparing their activities. Determined the enzyme properties of immobilized superoxide dismutase. Results showed superoxide dismutase on chitosan with glutaraldehyde as crosslinking agent showed highest activities and recovery rates. The temperature stability and the pH stability of the immobilized enzyme was much better than the free enzyme. And the immobilized enzyme also showed a good store stability. The immobilized superoxide dismutase can be prepared by crosslinking glutaraldehyde on chitosan,and possessed good properties. |
| |
| Keywords: | Superoxide dismutase Immobilized enzyme Chitosan |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
