| 高效液相色谱法-荧光检测器同时检测龙眼中多菌灵和噻菌灵残留 |
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| 引用本文： | 刘杰,杨春亮,查玉兵,林玲. 高效液相色谱法-荧光检测器同时检测龙眼中多菌灵和噻菌灵残留[J]. 广东农业科学, 2010, 37(3): 150-152 |
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| 作者姓名： | 刘杰 杨春亮 查玉兵 林玲 |
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| 作者单位： | 1. 海南大学食品学院,海南,海口,571000
2. 中国热带农业科学院农产品加工研究所,广东,湛江,524001;农业部食品质量监督检验测试中心(湛江),广东,湛江,524001 |
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| 摘 要： | 建立了同时检测龙眼中多菌灵和噻菌灵残留的高效液相色谱方法。样品经乙腈提取,硅胶柱净化,然后采用高效液相色谱法-荧光检测器测定,外标法定量。对样品前处理和色谱分离条件进行了研究和优化。两种杀菌剂在0.01μg/mL～2.00μg/mL范围内线性良好,相关系数分别为0.9990和0.9996。添加两个浓度水平(0.1、0.4μg/mL)两种杀菌剂在龙眼中的回收率均在95.7%～96.2%之间,相对标准偏差不大于2%。方法简便、快速、净化效果较好,可同时满足龙眼中两种杀菌剂残留的检验工作需要。
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| 关 键 词： | 龙眼 高效液相色谱法 荧光检测器 固相萃取 多菌灵 噻菌灵 |
Simultaneous determination of carbendazim and thiabendazole residues in longan by HPLC with fluorescence detector |
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| Abstract: | A reversed phase high performance liquid chromatography(HPLC) method has been developed for carbendazim and thiabendazole residues in Longan. Carbendazim and thiabendazole were extracted from Longan samples with acetonitrile. Extracts were cleaned up by Silica Gel column. Analytical samples were determined by HPLC and quantified by external standard method. The average recoveries spiked in longan at two concentration levels of 0.1,0.4 μg/mL ranged from 95.7% to 96.2% and the relative standard deviations were not greater than 2%.The method developed was easier, faster, and had better purification effect. It could can meet the requirements for simultaneous determination of carbendazim and thiabendazole in import and export inspection for Longan. |
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| Keywords: | Longan high performance liquid chromatography (HPLC) fluorescence detector solid-phase extraction(SPE) carbendazim(CBZ) thiabendazole(TBZ) |
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