22个非洲菊品种遗传多样性ISSR分析

[目的]从分子水平上了解非洲菊主栽品种的亲缘关系及遗传多样性,为选配非洲菊杂交育种亲本提供参考.[方法]设计100条ISSR引物,利用ISSR分子标记技术对外引的22个非洲菊品种进行遗传多样性及聚类分析.[结果]筛选的9个ISSR引物共扩增出139条清晰带,其中多态性带127条,多态性比率91.37%.品种间的遗传相似系数在0.5095~0.8889,平均为0.7230.UPGMA聚类分析结果可将供试材料分为4组,其中第1组又可分为两个亚组.[结论]非洲菊品种间具有较丰富的遗传多样性,花瓣及花心颜色可以作为区分非洲菊品种分类及分析亲缘关系的初步依据.

Genetic diversity analysis of 22 Gerbera jamesonii cultivars using ISSR markers

[Objective]The present experiment was conducted to study the genetic diversity and relationship amongst different Gerbera jamesonii cultivars in order to screen parents for hybrid breeding. [Method]Inter simple sequence repeat (ISSR) molecular markers were used to assess the genetic diversity amongst 22 G. jamesonii cultivars using 100 ISSR primers and cluster analysis was performed. [Result]Out of 100 primers, 9 primers were selected and 139 reproducible DNA bands were amplified, of which 127 bands (91.37%) were polymorphic. The coefficient of genetic similarity in different cultivars ranged from 0.5909 to 0.8889 with an average of 0.7230, showing that there was wide genetic diversity amongst different cultivars. Unweighted pair group method with arithmetic mean (UPGMA) was used to draw a dendrogram, according to which the cultivars were clustered into four groups, and the first group could be divided into two subgroups. [Conclusion]Wide genetic diversity amongst 22 G. jamesonii cultivars has been observed. Further, the morphological characteristics studies revealed that the color of petals and corolla may be used in preliminary classification and analysis of genetic relationship amongst various G. jamesonii cultivars.