不同培养条件对双孢蘑菇菌丝生长的影响

[目的]了解不同培养条件对双孢蘑菇菌株菌丝生长的影响,为筛选和选育适宜广西栽培的双孢蘑菇品种和确定适宜的栽培管理措施奠定基础.[方法]研究7个双孢蘑菇菌株的形态特征及不同温度、pH、光照、碳氮源对其菌丝生长的影响.[结果]在相同的培养条件下,菌株9506的菌丝生长速度最快,褐蘑菇最慢;双孢蘑菇菌丝生长的适宜温度为20~30C,最适温度为25℃;菌丝生长的适宜pH为4.0~9.0,最适pH为6.0~8.0;双孢蘑菇菌丝生长对光照条件要求不高;最适合菌丝生长的碳源是葡萄糖、氮源是酵母粉.[结论]7个双孢蘑菇品种间的遗传差异性不明显,在栽培生产中,应综合协调温度、pH、光照、碳氮源等因素的作用效果,以获得高产稳产.

Effects of different culture conditions on mycelium growth of Agaricus bisporus

[Objective]The present experiment was conducted to investigate the biological characteristics of different Agaricus bisporus strains in order to breed good quality Agaricus bisporus varieties suitable for cultivation in Guangxi and to develop proper management measures.[Method]The morphological characteristics of seven Agaricus bisporus strains were investigated and effects of temperature,pH,illumination,carbon and nitrogen sources on their mycelial growth were studied.[Result]Under the similar cultivation conditions,the strain 9506 showed the highest mycelial growth rate,while it was the lowest in portobello mushroom.The appropriate temperature for mycelial growth of Agaricus bisporus ranged from 20-30℃ with the optimal temperature being 25℃.The suitable pH for its mycelial growth was 4.0-9.0 with the best pH range between 6.0-8.0.Illumination was found to slightly affect the mycelial growth.The optimal carbon and nitrogen sources were glucose and yeast powder,respectively.[Conclusion]No significant genetic differences were observed amongst seven Agaricus bisporus species.Temperature,pH,illumination,carbon and nitrogen sources should be taken into account during cultivation to attain high and steady yield.