| 柔嫩艾美耳球虫长春株克隆的建立 |
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| 引用本文： | 魏峰,张西臣,赵永军,李春雨,李建华,尹继刚,吴涛. 柔嫩艾美耳球虫长春株克隆的建立[J]. 吉林农业大学学报, 2006, 28(5): 570-573 |
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| 作者姓名： | 魏峰 张西臣 赵永军 李春雨 李建华 尹继刚 吴涛 |
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| 作者单位： | 吉林大学畜牧兽医学院,长春,130062;吉林农业大学生物技术学院,长春,130118;吉林大学畜牧兽医学院,长春,130062;吉林大学畜牧兽医学院,长春,130062;内蒙古民族大学动物科技学院,通辽,028042 |
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| 摘 要： | 从柔嫩艾美耳球虫长春株(由单卵囊接种建立)挑选出单孢子囊,制备成单孢子囊胶囊,给鸡接种后建立5个克隆,传代后提取DNA,用已筛选的13条随机引物进行PCR扩增。结果表明:每个克隆均获得RAPD图谱,有特异条带。5个克隆中C2和C3相似值为0.993 1,C4和C5相似值为0.992 7,C1与C2和C4的相似值分别为0.931 6和0.875 3。鉴别结果表明C1为1个克隆,C2和C3为1个克隆,C4和C5为1个克隆,说明柔嫩艾美耳球虫卵囊中的4个孢子囊的基因组成不一致。
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| 关 键 词： | 柔嫩艾美耳球虫 克隆 RAPD 特异片段 |
| 文章编号： | 1000-5684（2006）05-0570-04 |
| 收稿时间： | 2005-10-16 |
| 修稿时间： | 2005-10-162006-02-26 |
Establishment and Identification of Clones from Changchun Strain of Eimeria tenella |
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| WEI Feng, ZHANG Xi-chen, ZHAO Yong-jun, LI Chun-yu, LI Jian-hua,et al.. Establishment and Identification of Clones from Changchun Strain of Eimeria tenella[J]. Journal of Jilin Agricultural University, 2006, 28(5): 570-573 |
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| Authors: | WEI Feng ZHANG Xi-chen ZHAO Yong-jun LI Chun-yu LI Jian-hua et al. |
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| Affiliation: | 1. College of Animal Husbandry and Veterinary Medicine, Jilin University, Changchun 130062, China; 2. College of Biotechnology, Jilin Agricultural University, Changchun 130118, China; 3. Inner Mongolia University for Nationalities, Tongliao 028042, China |
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| Abstract: | Five different clones,established by a single sporocyst infection from Changchun strain of Eimeria tenella,were studied by Random Amplified Polymorphic DNA(RAPD) with 13 screened random primers.The result showed that the RAPD map and the especial fragment of DNA were obtained.Among the five clones,SI of C2 and C3,C4 and C5,C1 and C2,and C1 and C4 is 0.993 1,0.992 7,0.931 6,and 0.835 7 respectively.These results showed that C1 is a clone, C2 and C3 is a clone,and C4 and C5 is a clone respectively,indicating that the genome of the 4 sporocysts in E.tenella oocyst is different. |
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| Keywords: | Eimeria tenella clone RAPD especial fragment |
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