低聚果糖诱导的奶牛急性蹄叶炎肝和骨骼肌中AMPK和GLUT及相关基因的变化

旨在探究奶牛急性蹄叶炎能量代谢的变化，本研究选用12头健康中国荷斯坦奶牛，随机分为两组(n=6): 试验组(OF组)用17 g·kg^-1体重的低聚果糖溶解在2 L·100 kg^-1体重的清水中灌服，对照组(CON组)灌服等量清水，72 h之后对奶牛进行安乐死，采集肝、肌肉组织，进行Western blot试验和实时荧光定量PCR试验，检测肝、肌肉组织的能量变化和葡萄糖转运情况，指标：葡糖糖转运蛋白1和4(GLUT-1、GLUT-4)，三磷酸腺苷激酶(AMPK)以及相关因子(PPAR-γ、PGC1-α、PEPCK)。结果表明：在肝组织中，OF组AMPK和蛋白的表达量显著增加，但P-AMPK/AMPK的比值极显著下降，而GLUT-1的蛋白和基因、PPAR-γ、PGC1-α和PEPCK基因的表达量无显著变化；在肌肉组织中，OF组AMPK基因和蛋白表达量无显著的变化，但P-AMPK/AMPK的比值显著下降，GLUT-4基因和蛋白表达量显著下降，同时PPAR-γ、PEPCK基因的表达量极显著升高，但PGC-1-α的基因表达量无显著的变化。综上：奶牛急性蹄叶炎可能会抑制肌肉组织的能量代谢和葡萄糖转运的能力，但对肝的影响不大。

Changes of AMPK,GLUT and Related Genes in Liver and Skeletal Muscle of Dairy Cows with Acute Laminitis Induced by Oligofructose

In order to investigate the changes in energy metabolism during the course of acute laminitis in dairy cows, 12 healthy Chinese Holstein cows were used in this study, they were randomly devided into two groups (n=6): The experimental group (OF group) was treated with 17 g·kg^-1 body weight of oligofructose dissolved in 2 L·100 kg^-1 body weight of water, and the control group (CON group) was given the same amount of water, and 72 hours later cows were euthanized to collect liver and muscle tissues, and Western blot experiments and real-time fluorescent quantitative PCR experiments were performed to detect energy changes and glucose transport in the liver and muscle tissues. Indicators: glucose transporter 1 and 4 (GLUT-1, GLUT- 4), 5'-adenosine monophosphate-activated protein kinase, AMPK and related factors (PPAR-γ, PGC1-α, PEPCK). Results were as follows: In liver tissues, the expression of AMPK gene and AMPK protein of OF group increased significantly, but the ratio of P-AMPK/AMPK decreased extremely significantly, while the protein and gene of GLUT-1, PPAR-γ gene, PGC1α gene and PEPCK gene no significantly change in expression; in muscle tissue, the protein expression of AMPK gene and AMPK in the OF group did not change significantly, but the ratio of P-AMPK/AMPK decreased significantly, the expression of GLUT-4 gene and protein decreased significantly, and the expression levels of PPAR-γ and PEPCK genes increased significantly, but there was no significant change in the expression levels of PGC1-α genes. Acute laminitis in dairy cows may inhibition the energy metabolism and glucose transport ability of muscle tissue, but has little effect on the liver.