| 用CTAB和SDS法简单快速提取拉曼被孢霉DNA的通用方法 |
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| 引用本文： | 孙继民,王卫卫,侯小娟,张儒.用CTAB和SDS法简单快速提取拉曼被孢霉DNA的通用方法[J].湖南农业科学,2007(6):61-63. |
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| 作者姓名： | 孙继民 王卫卫 侯小娟 张儒 |
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| 作者单位： | 1. 湖南省土壤肥料研究所,湖南,长沙,410125
2. 西北大学生科学学院,陕西,西安,710069 |
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| 基金项目： | 陕西省教育厅专项资助(01jk099) |
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| 摘 要： | 在植物及微生物DNA提取方法的基础上,提出了一种分别用CTAB和SDS试剂简单、快速、安全、优质提取拉曼被孢霉DNA的通用步骤。该方法提取时间短、效率高、产品较纯,通过紫外分光光度计检测和凝胶电泳分析,提取的DNA分子量可达21.2 kb以上,其纯度可直接用于PCR扩增等分子生物学方面的研究。
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| 关 键 词： | 拉曼被孢霉 DNA提取 PCR扩增 凝胶电泳 |
| 文章编号： | 1006-060X（2007）06-0061-03 |
| 收稿时间： | 2007-04-25 |
| 修稿时间： | 2007年4月25日 |
A Simple,Rapid and Universal Method of Mortierella Ramanni Ana DNA Extraction with CTAB and SDS |
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| SUN Ji-min,WANG Wei-wei,HOU Xiao-juan,Zhang-ru.A Simple,Rapid and Universal Method of Mortierella Ramanni Ana DNA Extraction with CTAB and SDS[J].Hunan Agricultural Sciences,2007(6):61-63. |
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| Authors: | SUN Ji-min WANG Wei-wei HOU Xiao-juan Zhang-ru |
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| Abstract: | This paper summarized a simplified,rapid,safe and quality method for extracting cell DNA from Mortierella ramanniance with CTAB and SDS,which was based largely on the isolation method of plants and microorganisms.The application showed the method was quick,effective and highpurity which the crude isolation DNA did not need to be purified and could be directly used to PCR analysis being diluted,as well as their OD260/280 and OD260/230.The quality of the extraction might be satisfied to the need of Molecular analysis.The extracted products had a main DNA bands more than 22.5 Kb in molecular length,which could be observed through agarose gel electrophoresis. |
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| Keywords: | Mortierella ramanniana DNA extraction PCR amplification agarose gel electrophoresis |
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