臭氧氧化猪场处理尾水对苦草(Vallisneria spiralis)抗氧化系统的影响

为了探索臭氧氧化技术应用于畜禽养殖废水排放前的预处理对环境可持续发展的影响,用不同浓度臭氧氧化处理经生化工艺处理后的猪场尾水,以苦草为试验材料,从活性氧代谢和抗氧化系统的角度,探讨了沉水植物在臭氧氧化猪场处理尾水中的适应性以及臭氧氧化技术的应用对水生植物的影响。结果表明:与未经臭氧氧化处理相比,臭氧氧化后(AO1 10 mg·L~(-1)、AO2 30 mg·L~(-1)、AO3 50 mg·L~(-1))猪场处理尾水中苦草的过氧化氢(H_2O_2)含量有升高趋势;AO2处理中苦草的抗坏血酸(AsA)-谷胱甘肽(GSH)循环对H_2O_2调节起重要作用,苦草的抗坏血酸过氧化物酶(APX)活性、AsA和GSH含量增加,从而使生物量增加;AO3处理中苦草AsA含量降低,H_2O_2与抗氧化物质之间没有达到平衡,从而使生物量降低。结果显示,试验条件下,30 mg·L~(-1)的臭氧氧化应用于猪场处理尾水,能增强沉水植物的抗氧化系统,促进生长,从而对水生生态系统有调节作用。

Effects of ozonation of piggery tail water on the antioxidant system in Vallisneria spiralis

Ozonation is becoming more prevalent as a pretreatment in the dispose of livestock wastewater, there are growing concerns on the effects of ozonation technology on sustainable development of environment.In this research, after the piggery tail water from biological pond system was further treated by ozonation, the effects of ozonized piggery tail water on the health of aquatic ecosystems were investigated in the terms of reactive oxygen species and antioxidant system of Vallisneria spiralis. The results showed that compared with the un-ozonized tail water, ozonation(AO1 10 mg·L^-1, AO2 30 mg·L^-1, AO3 50 mg·L^-1) increased hydrogen peroxide(H₂O₂) content in Vallisneria spiralis. AO2 treatment increased ascorbate peroxidase(APX) activity, ascorbic acid(AsA) and glutathione(GSH) content and the biomass, indicating the important role of AsA-GSH cycle in regulating H₂O₂ levels. However, AO3 treatment decreased AsA content, broke the balance between H₂O₂ and antioxidants and resulted in the decreased biomass of Vallisneria spiralis. Our results suggested that, 30 mg·L^-1 is the optimum ozone concentration to treat piggery tail water, by which it could promote the antioxidant system and growth of submerged macrophytes, and very helpful for the maintenance of healthy aquatic ecosystem.