| 三种牡丹组织培养比较研究 |
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| 引用本文： | 范小峰,郭小强,马世荣. 三种牡丹组织培养比较研究[J]. 北方园艺, 2010, 0(4): 132-134 |
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| 作者姓名： | 范小峰 郭小强 马世荣 |
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| 作者单位： | 陇东学院,生命科学系,甘肃,庆阳,745000;陇东学院,生命科学系,甘肃,庆阳,745000;陇东学院,生命科学系,甘肃,庆阳,745000 |
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| 基金项目： | 甘肃省科技攻关计划资助项目(2GS035-A41-001-06);;甘肃省教育厅研究生导师科研资助项目(0701-23) |
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| 摘 要： | 以3种牡丹的茎尖为外植体,研究不同品种牡丹茎尖在不同激素配比条件下,愈伤组织和丛生芽的诱导、芽的生长及生根情况。结果表明:适合3种牡丹丛生芽产生的最佳培养基,紫斑牡丹为MS+6-BA 0.5 mg/L+IBA 0.2 mg/L+GA30.1 mg/L+Vc 100 mg/L,青心白为MS+6-BA 0.5 mg/L+IBA 0.5 mg/L+CH 100 mg/L,朝霞为MS+6-BA 1.0 mg/L+IBA 0.5mg/L+CH 100 mg/L+Vc 100 mg/L;适合3种牡丹生根的培养基为1/2MS+IAA 1.0 mg/L+IBA 2.0 mg/L+NAA 0.2 mg/L,其中,朝霞生根效果最佳,生根率可达82.35%,紫斑牡丹和青心白分别为75%和50%;活性炭和Vc可有效防止褐化的发生。
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| 关 键 词： | 牡丹 茎尖 组织培养 快速繁殖 |
Comparative Studies on The Tissue Culture of Three Species of Peony |
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| FAN Xiao-feng,GUO Xiao-qiang,MA Shi-rong. Comparative Studies on The Tissue Culture of Three Species of Peony[J]. Northern Horticulture, 2010, 0(4): 132-134 |
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| Authors: | FAN Xiao-feng GUO Xiao-qiang MA Shi-rong |
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| Affiliation: | Department of Life Science;Longdong University;Qingyang;Gansu 745000 |
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| Abstract: | The stem apex of three peony were used to research induction of callus and cluster buds,buds growth and rooting in the compounding proportions of different hormones.The results indicated that the optimal medium for cluster buds inducing of three peony,Paeonia rockii was MS+6-BA 0.5 mg/L+IBA 0.2 mg/L+GA30.1 mg/L+Vc 100 mg/L,cv.QingXinBai was MS+6-BA 0.5 mg/L+IBA 0.5 mg/L+CH 100 mg/L,cv.Zhao Xia was Ms+6-BA 1.0 mg/L+IBA 0.5 mg/L+CH 100 mg/L+Vc 100 mg/L;the optimal medium for rooting was 1/2MS+IAA 1.0 mg/L+IBA... |
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| Keywords: | Paeonia suffruticosa stem apex tissue culture rapid propagation |
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