| 橡胶树多主棒孢病菌遗传多态性分析 |
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| 引用本文： | 黄贵修,刘先宝,高宏华,蔡吉苗,时涛,张科立.橡胶树多主棒孢病菌遗传多态性分析[J].热带作物学报,2008,29(6):777-780. |
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| 作者姓名： | 黄贵修 刘先宝 高宏华 蔡吉苗 时涛 张科立 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所,海南省热带农业有害生物检测监控重点实验室,海南,儋州,571737 |
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| 基金项目： | 科技部国家科技支撑计划项目，科技部农业科技成果转化资金项目，农业部公益行业科研专项基金，中央级公益性科研院所基本科研业务费专项资金资助项目 |
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| 摘 要： | 利用ISSR（简单序列重复区间扩增多态性）和RAPD（随机扩增多态DNA）技术对供试的18个多主棒孢菌株进行了遗传多态性分析，研究结果表明，2种分子标记技术均能揭示橡胶多主棒孢病菌的遗传多态性。同时利用P距离模型构建了18个多主棒孢菌株的UPGMA聚类分析树状图，根据2种技术构建的UPG—MA聚类树状图可将供试菌株分为3个主要类群。ISSR类群和RAPD类群与地理来源均不存在明显的关系。
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| 关 键 词： | 橡胶 多主棒孢 ISSR RAPD |
Assessment of Genetic Diversity of Corynespora cassiicola on Rubber Tree by RAPD and ISSR Analysis |
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| HUANG Guixiu,LIU Xianbao,GAO Honghu,CAI Jimiao,SHI Tao and ZHANG Keli.Assessment of Genetic Diversity of Corynespora cassiicola on Rubber Tree by RAPD and ISSR Analysis[J].Chinese Journal of Tropical Crops,2008,29(6):777-780. |
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| Authors: | HUANG Guixiu LIU Xianbao GAO Honghu CAI Jimiao SHI Tao and ZHANG Keli |
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| Institution: | Hainan Key Laboratory for Detection and Monitoring of Tropical Agricultural Pests/Environment and Plant Protection Institute, CATAS;Hainan Key Laboratory for Detection and Monitoring of Tropical Agricultural Pests/Environment and Plant Protection Institute, CATAS;Hainan Key Laboratory for Detection and Monitoring of Tropical Agricultural Pests/Environment and Plant Protection Institute, CATAS;Hainan Key Laboratory for Detection and Monitoring of Tropical Agricultural Pests/Environment and Plant Protection Institute, CATAS;Hainan Key Laboratory for Detection and Monitoring of Tropical Agricultural Pests/Environment and Plant Protection Institute, CATAS;Hainan Key Laboratory for Detection and Monitoring of Tropical Agricultural Pests/Environment and Plant Protection Institute, CATAS |
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| Abstract: | Eighteen isolates of Corynespora cassiicola collected from rubber trees (Hevea brasiliensis) were analyzed with random amplified polymorphic DNA (RAPD) and internal simple sequence repeat (ISSR) techniques. The results showed that both of these two molecular markers can reveal the genetic diversity of the isolates of C. cassiicola. The UPGMA cluster analysis with p-distance similarity coefficient was used to generate the dendrograms showing relationship between the 18 isolates, based on which the 18 isolates were clustered into 3 groups. However, both ISSR and RAPD clustering groups had no obvious correlation with the geographical origin of the isolates. |
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| Keywords: | ISSR RAPD |
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