基因芯片技术在猪病毒性疾病诊断中的应用

基因芯片是研究生物大分子功能的新技术,具有高通量、高度平行性、高度自动化的特点。它是通过点样法或原位合成法把大量基因探针或基因片段按特定的排列方式固定在硅片上形成致密有序的DNA分子点阵,按碱基配对的特性与样品DNA杂交,然后通过计算机进行解读和分析,以获取大量信息。在对动物传染病病原体的研究中,基因芯片技术已应用于病原体检测、基因分型、表达谱的分析等。论文就其定义、作用机理、分类、在动物病毒性传染病方面的应用及其存在的问题和发展前景进行了综述。

Application of Gene Chip Technology in Diagnosis of Swine Viral Diseases

Gene chip,which was characterized by its high throughput,highly parallel and highly automatic,was a newly developed technology in recent years.Light-directed in situ oligonucleotide synthesis or microarray printing technique was adopted in its basic process in order to generate arrays of large amounts of probes on the surface of small substrates such as silicon chips,which was hybridized to the amplified and labeled samples.After scanning and analyzing,the signals provided hereditary information on the samp...