金霉素和4-差向金霉素对油菜生长的影响及其在幼苗体内的积累

以油菜为材料,研究不同浓度金霉素（chlortetracycline,CTC）和代谢物差向金霉素（4-epi-chlortetracycline,ECTC）胁迫对其发芽、生长和抗氧化酶活性的影响,以及CTC和ECTC在幼苗体内的积累。结果表明：随着处理浓度的升高,种子发芽率、发芽势没有显著差异,但株高和根长受到明显抑制;在1～30mg.L-1处理浓度下,CTC和ECTC诱导的幼苗体内抗氧化酶SOD、POD、CAT活性均高于对照组;幼苗体内CTC、ECTC积累含量随着处理浓度的升高而增加,表明CTC和ECTC可在油菜幼苗体内积累,这可能产生潜在食品安全风险。

Effects of Chlortetracycline and 4-epi-chlortetracycline on the Growth of Rape and Its Accumulation in Seedling

Effects of different concentrations of chlortetracycline （CTC） and 4-epi-chlortetracycline （ECTC） on the growth and the activities of antioxidant enzymes in rape and the accumulation of CTC and ECTC in seedlings were studied by using water culture method. The results showed that seedling height and root length significantly reduced, compared to the control, and the root elongation could be recommended as a sensitive indicator to assess crops eco-toxicity of CTC and ECTC. The activities of antioxidant enzymes as superoxide dismutase（SOD）, peroxidase（POD ） and catalase（CAT） induced by CTC and ECTC at concentration ranges of 1-30 mg.L^-1 were higher than the control. When rape seedling was stressed by 1 mg. L^-1 and 10 mg. L^-1 of CTC and ECTC, the activities of SOD and POD were enhanced, but declined by 30 mg .L^-1. SOD, POD removed oxygen free radical in coordination with CAT at concentration levels of 1 mg .L^-1 and 10 mg. L^-1 of CTC and ECTC. However, at a concentration of 30mg.L^-1, CTC and ECTC would cause certain oxidative damage on rape seedling. The difference of antioxidant enzyme system suggested that ability of rape seedling anti-CTC stress was stronger than anti-ECTC. With the concentration of CTC and ECTC increased, the contents of them accumulated in seedlings were increased, which could result in a potential food safety risk.