黑曲霉产木聚糖酶的固态发酵条件优化 |
| |
引用本文: | 周晨妍,张金华,马雪婷,周锋,周克楠,丰慧根. 黑曲霉产木聚糖酶的固态发酵条件优化[J]. 安徽农业科学, 2010, 38(21): 11052-11054. DOI: 10.3969/j.issn.0517-6611.2010.21.007 |
| |
作者姓名: | 周晨妍 张金华 马雪婷 周锋 周克楠 丰慧根 |
| |
作者单位: | 新乡医学院生命科学技术系,河南省医学遗传学与分子靶向药物高校重点实验室培育基地,河南新乡,453003;新乡医学院护理学院,河南新乡,453003 |
| |
摘 要: | [目的]研究培养基组成和培养条件对黑曲霉固态发酵产木聚糖酶的影响。[方法]以木聚糖酶酶活为评价指标,利用单因素试验和L9(34)正交试验考察摇瓶发酵条件下培养基组成、pH值、培养时间、培养温度和接种量对黑曲霉产生木聚糖酶的影响。[结果]最佳培养基组成为:麸皮与玉米芯质量比5∶3,(NH4)2SO4、KH2PO4、CaCl2和MgSO4相对于固体料的质量百分数分别为1.0%、0.2%、0.1%和0.1%),料液比1∶1.7;最优培养条件为:pH值7.5、培养温度28℃、培养时间60h,其间翻曲2~3次;在此工艺条件下,木聚糖酶的活力可达14698.21IU/g。[结论]该优化条件为木聚糖酶的工业化生产和应用提供了依据。
|
关 键 词: | 固态发酵 木聚糖酶 黑曲霉 正交试验 |
Optimization of Culture Conditions for Xylanase Production by the Solid-state Fementation with Aspergillus niger |
| |
Affiliation: | ZHOU Chen-yan et al(Department of Life Science and Technology,The Key Laboratory of Medical Genetics and Molecular Targeting Drugs,Henan Province,Xinxiang Medical University,Xinxiang,Henan 453003) |
| |
Abstract: | [Objective]To investigate the solid fermentation for xylanase production by Aspergillus niger.[Method] The single-factor tests and orthogonal experiment were employed to evaluate the optimal fermentation conditions for xylanase production by solid-state fermentation with Aspergillus niger.[Result]The optimum conditions for producing xylanase are as follows:the ratio of wheat bran:cob in medium 5∶3,(NH4)2SO4 1.0 %,KH2PO4 0.2%,CaCl2 0.1%,MgSO4 0.1%,the ratio of solid to fluid 1∶1.7,the inoculum 1.0%,the initial pH 7.5,the temperature 28 ℃,and the time for fermentation 60 h.Under those conditions,the enzyme activity of xylanase roduced by the strain reached 14 698.21 IU/g dry medium.[Conclusion]This study was meaningful for the production and application of xylanase in industry. |
| |
Keywords: | Solid-state fermentation Xylanase Aspergillus niger Orthogonal experiment |
本文献已被 维普 万方数据 等数据库收录! |
|