| 山豆根组培快繁技术优化的研究 |
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| 引用本文： | 姚绍嫦,凌征柱,蓝祖栽,马小军.山豆根组培快繁技术优化的研究[J].北方园艺,2011(6):136-139. |
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| 作者姓名： | 姚绍嫦 凌征柱 蓝祖栽 马小军 |
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| 作者单位： | 1. 广西药用植物园,广西南宁,530023
2. 广西药用植物园,广西南宁,530023;中国医学科学院药用植物研究所,北京100193 |
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| 基金项目： | 国家科技重大专项资助项目，国家发改委中药材扶持资金资助项目 |
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| 摘 要： | 以药用植物山豆根为材料,通过探讨不同部位、基本培养基、6-BA浓度和继代代数对有效繁殖系数和成苗质量的影响,以优化山豆根的组培快繁技术。结果表明:顶芽茎段为诱导的最佳部位;改良B5低氮盐,为最适基本培养基;6-BA 2.0 mg/L对芽分化和苗的质量较优;较为合适的继代代数为10~15代。
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| 关 键 词： | 山豆根 试管苗 有效繁殖系数 成苗质量 |
Optimization of Tissue Culture on Sophora tonkinensis Gapnep. |
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| YAO Shao-chang,LING Zheng-zhu,LAN Zu-zai,MA Xiao-jun.Optimization of Tissue Culture on Sophora tonkinensis Gapnep.[J].Northern Horticulture,2011(6):136-139. |
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| Authors: | YAO Shao-chang LING Zheng-zhu LAN Zu-zai MA Xiao-jun |
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| Institution: | YAO Shao-chang1,LING Zheng-zhu1,LAN Zu-zai1,MA Xiao-jun1,2(1.Guangxi Botanical Garden of Medicinal Plant,Nanning,Guangxi 530023,2.Institute of Medicinal Plant,Chinese Academy of Medical Science,Beijing 100094) |
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| Abstract: | The effect of several factors such as different positions,basic media,6-BA concentration and subculture times on effective propagation coefficient and seedling quality was discussed in this paper by using tube seedlings of Sophora tonkinensis Gapnep.The results showed that the stem with terminal bud was the best position.Medium which had lower concentration of nitrogen was beneficial to propagation and seedling quality,so that improved B5 medium was the most suitable one.When 6-BA concentration was 6-BA 2.0... |
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| Keywords: | Sophora tonkinensis Gapnep tube seedling effective propagation coefficient seedling quality |
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