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| 栽培菊感染菊花白锈病后体内防御酶活性的研究 | |
| 引用本文： | 祝朋芳,赵妮拉,段玉玺.栽培菊感染菊花白锈病后体内防御酶活性的研究[J].北方园艺,2011(6):77-80. |
| 作者姓名： | 祝朋芳 赵妮拉 段玉玺 |
| 作者单位： | 1. 沈阳农业大学林学院,辽宁,沈阳,110866;沈阳农业大学植物保护博士后流动站,辽宁,沈阳,110866 2. 沈阳农业大学林学院,辽宁,沈阳,110866 3. 沈阳农业大学植物保护博士后流动站,辽宁,沈阳,110866 |
| 基金项目： | "十一五"国家科技支撑计划资助项目，沈阳农业大学博士后研究基金 |
| 摘 要： | 对切花菊抗、感品种分别接种菊花白锈病病原菌,肉眼观察及显微鉴定其症状变化,并采用分光光度法,定期测定菊花叶片内PAL、PPO及POD的活性并进行比较分析,探讨其与抗病性的关系。结果表明:在接种后15 d内,C039、B86均未出现明显症状,而C002于接种后第10天肉眼可见冬孢子堆,B76于接种后第11天肉眼可见冬孢子堆;随着病原菌的侵染,与对照相比,切花及盆栽的抗、感菊花品种的PAL、PPO及POD活性均呈不同程度的升高,活性提高幅度及变化规律因酶而异,就同一种酶而言,抗病品种酶活性较感病品种增高幅度大;在菊花与菊花白锈病病原菌互作过程中,菊花白锈病的潜育期长短因品种而异,防御酶活性的动态变化与抗病性密切相关,这3种防御酶活性峰值高低及出现的早晚可作为菊花白锈病早期抗性鉴定的重要生理指标。 |
| 关 键 词： | 栽培菊 菊花白锈病 苯丙氨酸解氨酶 多酚氧化酶 过氧化物酶 |
Study of Defense Enzymes Activities on Cultivation Chrysanthemum Infected by Chrysanthemum White Rust |
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| ZHU Peng-fang,ZHAO Ni-la,DUAN Yu-xi.Study of Defense Enzymes Activities on Cultivation Chrysanthemum Infected by Chrysanthemum White Rust[J].Northern Horticulture,2011(6):77-80. | |
| Authors: | ZHU Peng-fang ZHAO Ni-la DUAN Yu-xi |
| Institution: | ZHU Peng-fang1,2,ZHAO Ni-la1,DUAN Yu-xi2(1.College of Forestry,Shenyang Agricultural University,Shenyang,Liaoning 110866,2.Plant Protection Center for Post-doctoral Studies,Liaoning 110866) |
| Abstract: | We identified symptoms by eyes and under microscope,detected four defense enzymes activity through UV-spectrophotometry termly after inoculating the pathogen to different resistant cut-flowers(C039,C002),potted chrysanthemum(B86,B76) varieties.We focused on activity changes of phenylalanine ammonia lyase(PAL),polyphenol oxidase(PPO),and peroxidase(POD) and after inoculation in order to find relationships of different varieties to resistance.The results showed that we had not found any symptoms on disease-re... |
| Keywords: | cultivation chrysanthemum chrysanthemum white rust phenylalanine ammonia lyase polyphenol oxidase peroxidase |
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