| 甜椒叶绿体小分子量热激蛋白CaHSP26增强拟南芥响应高温胁迫 |
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| 引用本文： | 贺立龙,高娜娜,单忠英,郭尚敬.甜椒叶绿体小分子量热激蛋白CaHSP26增强拟南芥响应高温胁迫[J].北方园艺,2011(6):140-143. |
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| 作者姓名： | 贺立龙 高娜娜 单忠英 郭尚敬 |
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| 作者单位： | 聊城大学生命科学学院,山东聊城,250059 |
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| 基金项目： | 山东省优秀中青年专家基金资助项目，国家自然科学基金资助项目 |
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| 摘 要： | 为了探讨小分子量热激蛋白(CaHSP26)与植物抗热逆境的相互关系,以转CaH-SP26的拟南芥植株为研究材料,热激后测定野生型植物和转基因植物中电导率(REC)、丙二醛(MDA)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)以及脯氨酸和可溶性糖的含量。结果表明:热激后转基因拟南芥的REC、MDA只相当于野生型的73.78%6、1.14%;CAT、APX比野生型高115.10%、125.77%;脯氨酸和可溶性糖的含量比野生型高106.12%、53.31%。HSP26蛋白增强了拟南芥抗高温的能力。
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| 关 键 词： | CaHSP26 拟南芥 高温胁迫 |
Pepper Chloroplast Small Heat Shock Proteins CaHSP26 Enhanced The Ability of Anti-high Temperature Stress in Arabidopsis |
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| HE Li-long,GAO Na-na,SHAN Zhong-ying,GUO Shang-jing.Pepper Chloroplast Small Heat Shock Proteins CaHSP26 Enhanced The Ability of Anti-high Temperature Stress in Arabidopsis[J].Northern Horticulture,2011(6):140-143. |
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| Authors: | HE Li-long GAO Na-na SHAN Zhong-ying GUO Shang-jing |
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| Institution: | HE Li-long,GAO Na-na,SHAN Zhong-ying,GUO Shang-jing(College of Life Science,Liaocheng University,Liaocheng,Shandong 250029) |
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| Abstract: | In order to investigate the relationship between small heat shock protein CaHSP26 and heat stress on plant.Arabidopsis thaliana(Columbia) transformed with CaHSP26 were used to analyze Conductivity(REC),malondialdehyde(MDA),Catalase(CAT),Ascorbate peroxidase(APX) and content of Proline and Soluble sugar in wild-type plants and transgenic plants after high temperature stress.The results suggested that REC,MDA content of transgenic Arabidopsis plant were only equivalent to wild-type at 73.78%、61.14%;CAT,APX were higher than wild-type to 115.10%,125.77%;content of Proline and soluble sugar were higher than the wild-type to 106.12%,53.31%.HSP26 protein in Arabidopsis enhanced the ability of anti-high temperature stress. |
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| Keywords: | CaHSP26 |
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