Infection of established cell lines with Campylobacter mucosalis |
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| Affiliation: | 2. University of South Australia, UniSA Clinical and Health Sciences, SA, Australia;3. School of Veterinary Science, The University of Queensland, QLD, Australia;4. Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia |
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| Abstract: | Campylobacter mucosalis (CM) and other Campylobacter spp. mainly from cases of the porcine proliferative enteropathies were used to infect cell line cultures. CM attached to and persisted intracellularly in PK (15), MDBK, BHK, HeLa, INT-407 and other cells; in PK (15) this infection resulted in a marked cytopathic effect and total destruction of the cell culture in 10 days. MDBK, INT-407, BHK and HeLa cultures were more resistant to the effects of infection. CM did not adhere to Vero, LLCMK2 or CEF cells and no cytopathic changes took place. The other Campylobacter spp tested did not attach to cell cultures and cell death occurred within 4 days. Attempts to passage CM were unsuccessful due to a decrease in the number of bacteria at each passage. |
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