3种寄主挥发物中引起旋幽夜蛾嗅觉反应的活性组分分析

为探究植物挥发物中引起旋幽夜蛾嗅觉电生理反应的活性组分, 本研究采用动态顶空吸附法收集藜、甜菜和棉花植株的挥发物, 利用气相色谱-触角电位联用仪(GC-EAD)和气相色谱-质谱联用仪(GC-MS)定性鉴定寄主挥发物中引起旋幽夜蛾嗅觉反应的活性组分。结果表明, 藜挥发物中有6种活性组分, 甜菜和棉花挥发物中各有5种, 其中乙酸叶醇酯、1, 2-二乙苯、对二乙苯、壬醛、二乙二醇丁醚为3种植物共有的挥发物组分, 异佛尔酮仅在藜挥发物中发现。触角电位(EAG)测定结果显示, 以上6种组分都能引起旋幽夜蛾雌雄成虫的EAG反应, 且随浓度增加EAG相对反应值增大。雌雄成虫对壬醛和异佛尔酮的EAG反应最强烈, 其次是乙酸叶醇酯和1, 2-二乙苯。在相同浓度条件下, 雌雄成虫对同种化合物的EAG反应无性别特异性。本研究为探明旋幽夜蛾定位寄主机制及开发行为调控产品提供基础。

Analysis of active components in three host volatiles eliciting olfactory responses in Scotogramma trifolii

To investigate the active components of plant volatiles eliciting olfactory electrophysiological responses in Scotogramma trifolii, volatile compounds from Chenopodium album, Beta vulgaris, and Gossypium hirsutum. were collected using the dynamic headspace adsorption method. The active components in the host volatiles were qualitatively identified using gas chromatography electroantennography (GC-EAD) and gas chromatography-mass spectrometry (GC-MS). The results revealed that there are six active components in the volatile emissions of C.album, and five active components each in sugar beet and cotton volatiles. Among these, five components were common across all three plant volatiles, namely trans-3-hexenyl acetate, 1, 2-diethylbenzene, 1, 4-diethylbenzene, nonanal, and diethylene glycol monobutyl ether. Additionally, isophorone was exclusively found in the volatiles of C.album. The antennal electrophysiological responses (EAG) of S.trifolii reveal that the six active components can all cause EAG responses in both male and female adults, and increasing with increasing concentration, and the strongest EAG responses were observed to nonanal and isophorone, followed by trans-3-hexenyl acetate and 1, 2-diethylbenzene. Furthermore, under the same concentration conditions, the EAG response of male and female adults to the same compound displayed no gender specificity. This study provides a basis for exploring the host localization mechanism of S.trifolii and for developing behavioral regulators.