| 桃潜隐花叶类病毒中国分离株P3的克隆与序列分析 |
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| 引用本文： | 徐文兴,王国平,何云蔚,洪霓. 桃潜隐花叶类病毒中国分离株P3的克隆与序列分析[J]. 植物病理学报, 2005, 35(4): 300-304 |
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| 作者姓名： | 徐文兴 王国平 何云蔚 洪霓 |
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| 作者单位： | 华中农业大学植物科技学院, 武汉 430070 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 本研究以提取的类病毒cRNA为模板,采用RT-PCR方法,从5株表现明显花叶症状的"雨花露"桃树上检测到桃潜隐花叶类病毒(PLMVd),并对其中的样品P3通过2次RT-PCR扩增,然后分别将扩增产物回收、克隆测序,确定了该分离株(命名为PLMVd-P3分离株)的全长核苷酸序列,其cDNA分子全长为337 nt,这与已经报道的PLMVd典型分离株基因组大小相似。序列比较分析结果表明,该分离株与已经报道的不同地区来源的其它分离株相似性为90%~96%。
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| 关 键 词： | 桃潜隐花叶类病毒 RT-PCR 克隆 序列 |
| 文章编号： | 0412-0914(2005)04-0300-05 |
| 修稿时间： | 2004-07-28 |
Cloning and nucleotide sequence analysis of PLMVd isolate P3 from China |
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| XU Wen-xing,WANG Guo-ping,HE Yun-wei,Hong Ni. Cloning and nucleotide sequence analysis of PLMVd isolate P3 from China[J]. Acta Phytopathologica Sinica, 2005, 35(4): 300-304 |
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| Authors: | XU Wen-xing WANG Guo-ping HE Yun-wei Hong Ni |
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| Affiliation: | Plant Science and Technology College of Huazhong Agricultual University, Wuhan 430070, China |
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| Abstract: | Peach latent mosaic viroid (PLMVd) was detected from five plants of peach cultivar "Yuhualu" showing typical mosaic symptom by RT-PCR.RT-PCR product of P_(3 )sample_( )was collected and cloned into the vector pMD18-T.Full-length cDNA of the viroid was sequenced.The result showed the isolate had a size 337 nt,similar to that of typical PLMVd variants.Nucleotide sequence of the isolate had similarities of 90%-96% with other reported isolates from different areas. |
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| Keywords: | RT-PCR |
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