过表达类光捕获蛋白3降低水稻籽粒中三烯生育酚含量

类光捕获蛋白3 (Light harvesting like protein 3, LIL3) 是光捕获复合物蛋白超家族 (Light harvesting complex, LHC) 的一员, 其特征是存在一个保守的 LHC motif 序列. 水稻 (Oryza sativa L.) LIL3 参与叶绿素、生育酚侧链的生物合成. 维生素 E 包括生育酚和三烯生育酚2种, 是一类重要的脂溶性化合物, 具有抗氧化活性. 为了探究 LIL3对水稻籽粒维生素E 含量的影响, 在日本晴（Oryza Sativa spp. Japonica.）中过表达 LIL3，得到了稳定的转基因株系. 分别对野生型和过表达植株穗长、每穗粒数、粒长、粒宽以及籽粒中的维生素 E 含量做了分析. 结果表明: 与野生型相比, LIL3过表达植株的穗长、粒长和粒宽没有明显变化, 每穗籽粒数略有所下降. 籽粒中三烯生育酚含量降低.

Overexpression of LIL3 reduces tocotrienol contents in rice grains

Light harvesting like protein 3 (LIL3) is a member of the light harvesting complex family (LHC), which is characterized by a conserved LHC motif sequence. Rice (Oryza sativa L.) LIL3 participates in the side chain biosynthesis of chlorophyll and tocopherol. Vitamin E, including tocopherols and tocotrienols, is an important fat soluble compound with antioxidant activity. In order to explore the effects of LIL3 on vitamin E contents in rice grains, LIL3 was overexpressed in Nipponbare （Oryza Sativa spp. Japonica.）, and a stable transgenic line was obtained. The panicle length, number of grains per panicle, grain length, grain width and the content of vitamin E in grains were analyzed, respectively, in wild type and overexpressing plants. The results showed that the panicle length, grain length and width of LIL3 overexpression plants did not change significantly, compared with those of the wild type. However, number of grains per panicle decreased slightly. In addition, the total contents of tocotrienols obviously decreased in the LIL3 overexpressing plants.