厂形叶幕赤霞珠葡萄果实苹果酸的代谢规律

[目的]研究厂形叶幕形赤霞珠葡萄果实苹果酸的积累代谢规律,为葡萄有机酸代谢调控的研究提供理论依据.[方法]在厂形树形的基础上,设置对照直立形叶幕及2种高度的厂形叶幕,检测赤霞珠果实成熟过程苹果酸等有机酸的变化及苹果酸代谢相关酶的活性变化.[结果]厂形叶幕与直立形叶幕相比,降低了果域环境温度0.8℃与1.5℃.厂形叶幕在...

Study on the Malate Metabolism of Cabernet Sauvignon Grape under Inverted L Shape Canopy Type

【Objective】 To study the accumulation and metabolism of malate in Cabernet Sauvignon grapes under the inverted L shape canopy type.【Method】 On the basis of the inversed L-VSP trellis, a VSP shape and two kinds of inverted L shape canopy types were set up to detect the changes of organic acids such as malate and the activities of malate metabolism related enzymes during the ripening of Cabernet Sauvignon fruits.【Result】 Two inverted L shape canopy types reduced the temperature of the fruit-zone microenvironment by 0.8℃ and 1.5℃ compared to the VSP shape canopy type. The inverted L shape canopy type could increased the malic acid and tartaric acid content, with ripe fruit having 0.338 mg/g and 0.365 mg/g of malic acid and 0.774 mg/g and 0.760 mg/g of tartaric acid, respectively. The H₁ canopy with higher leaf height is more conducive to the accumulation of malate than the lower H₂ canopy. The PEPC and NAD-MDH enzyme activities of the three canopy types' fruits showed varying degrees of positive correlation with fruit malate content, and NADP-ME enzyme activity showed varying degrees of negative correlation with fruit malate content.【Conclusion】 The simplified inversed L shape canopy setting can achieve the purpose of regulating malate accumulation and metabolism in Cabernet Sauvignon grapes, which provides the theoretical basis and empirical reference for the study of the regulation of grape organic acid metabolism.