桂花新品种‘胭脂红’不同发育阶段花瓣非靶向代谢组学分析

‘胭脂红’（Osmanthus fragrans‘Yanzhi Hong’）是近几年报道过的一个桂花新品种，其花色为特殊的胭脂红色。为探究‘胭脂红’的特殊性和潜在的应用价值，通过非靶向代谢组学的方法，对不同发育阶段（初花期S1、盛花期S2和末花期S3）‘胭脂红’花瓣中代谢物的种类和积累规律进行研究。结果表明，‘胭脂红’花瓣中共检测到304种代谢物。其中6种环烯醚萜类和1种黄酮类药用化学成分是首次在桂花中检测到；从S1到S3，苦杏仁苷的相对含量逐渐降低，连翘酯苷E、5-O-甲基维斯阿米醇苷、连翘酯苷和大车前苷的相对含量先下降后上升，桃叶珊瑚苷和金雀异黄酮的相对含量先上升后下降。所测代谢物中有34种与花香有关的香气成分，其中含量最高的是紫苏醇，其次是3,4-二羟基苯丙酸酯、香叶酸和茉莉酮酸甲酯等。KEGG富集分析显著富集到与花香有关的苯丙氨酸和α-亚麻酸代谢途径，以及与花色有关的类单萜生物合成途径。综合分析，‘胭脂红’有着特殊的药用化学成分、独特的香气成分组成和花色，作为桂花的新品种，有着重要的药物开发和经济利用价值。

Non-Targeted Metabolomics Analysis of the Petals of Osmanthus fragrans ’Yanzhi Hong’ in Different Developmental Phases

Osmanthus fragrans ’Yanzhi Hong’ is a new cultivar reported in recent years,whose petals color is specially carmine.In order to explore the particularity and potential application value of the cultivar ’Yanzhi Hong’,this study used non-targeted metabolomics methods to analyze the types and accumulation rules of petals metabolites at 3 developmental phases:primary blooming stage (S1),full blooming stage (S2) and late blooming stage (S3).The results showed that a total of 304 metabolites were detected in the petals.Among them,6 iridoids and 1 flavonoid were firstly detected in O.fragrans.From S1 to S3,the relative content of amygdalin gradually decreased; the relative contents of forsythoside E,5-O-methylvisammioside,forsythoside and plantamajoside decreased first and then increased; the relative contents of aucubin and genistein increased first and then decreased.There were 34 aroma components in the tested metabolites,in which the highest relative content was perillyl alcohol,followed by 3,4-dihydroxyphenylpropanoate,geranic acid and methyl jasmonate.According to the comprehensive analysis，as a new cultivar,O.fragrans ’Yanzhi Hong’,which has special medicinal chemical components,unique composition of aroma components and petals colors,has significant values in drug development and economic utilization.