基于近红外光谱反射技术的砀山酥梨花粉活力快速检测

[目的]研究基于近红外光谱反射技术的砀山酥梨花粉活力快速检测.[方法]以砀山酥梨花粉为试材.4℃下贮藏,以0、7、27、43与65 d贮藏后花粉为试材,用离体萌发法测定花粉活力,利用近红外光谱仪扫描花粉采集光谱数据.以修正偏最小二乘法(MPLS)对采集的100个梨花粉近红外反射光谱与花粉活力进行定标模型拟合,判别鉴定砀...

Determination of Dangshan Pear Pollen Vitality Using Near -Infrared Spectroscopy

【Objective】 The purpose of this study is to explore the feasibility of rapid detection of pollen viability of Dangshan pear by near-infrared spectroscopy. Pollens are male reproductive cells of botanical stamen and male gametophyte of higher plant, which not only carry genetic information of species, but also contain essential nutritious substance of breeding new life. When cross breeding is proceeded, artificial supplementary pollination was taken to solve the problems of different fruit species flowering mismatch and the cross difficulty between varieties of different places. 【Methods】 At storage temperature 4℃, Dangshan pear pollen was taken as test material and after 0 days, 7 days, 27 days,43 days and 65 days, pollen vigor was determined by in vitro germination method, and then, the spectral data were collected by scanning pollen with near infrared spectrometer; A modified partial least square method (MPLS) was used to fit the calibration model of the near infrared reflectance spectra and pollen viability of 100 pear pollen collected. At the same time, the pollen of Dangshan pear was identified by near infrared spectrum. 【Results】 The results showed that the pollen vigor of Dangshan pear was 70.6%, 60.3%, 57.1%, 53.2% and 34.1% respectively. From the beginning, the storage vigor droppped from 70.6% to 34.1%, the vigor decreased by 52%, and the pollen vigor varied significantly among them. Moreover, the research also revealed the calibration model between feature information of pollen viability and the near-infrared spectrogram by multiple linear regression. The results showed that the calibration model whose correlation coefficient R ² was 0.985, the cross validation correlation coefficient 1-VR was 0.952,9, the standard cross validation activity by SECV was 2.5%. Taking log lg (R1540R1956) as independent variable after arithmetic addition of spectral reflectance, the fitted ExpAssoc equation was R ² 0.999,8, and the pollen viability had a functional relationship with its spectral reflectance.【Conclusion】 It is feasible to determine the pollen vigor of Dangshan pear by near infrared reflection technique.