补饲不同剂量竹叶提取物对泌乳期伊犁马血液生化指标的影响

【目的】研究补喂不同剂量竹叶提取物对泌乳期伊犁马抗氧化指标、血清蛋白、免疫指标和激素指标的影响,为分析竹叶提取物能够作为调节伊犁马母马抗氧化、免疫性能及激素的新型无害添加剂提供理论依据。【方法】选取伊犁哈萨克自治州昭苏马场同一草场放牧条件下体况相近的24匹母马作为研究对象,随机分为4组,每组6匹马;对照组不添加竹叶提取物,试验Ⅰ、Ⅱ、Ⅲ组分别添加10、20、30 g/d,饲喂周期为60 d,在第0、15、30、45、60 d通过采集母马的颈静脉血液分析不同补饲梯度血液生化指标的差异。【结果】(1)血液中抗氧化因子:试验Ⅲ组MDA的浓度极显著低于对照组及试验Ⅰ组(P<0.01)。(2)血清蛋白:试验Ⅱ组TP浓度显著低于试验Ⅰ、Ⅲ组(P<0.05),极显著低于对照组(P<0.01);试验Ⅰ组ALB浓度显著低于对照组(P<0.05),极显著低于试验Ⅲ组(P<0.01);试验Ⅱ组GLB浓度极显著低于对照组、试验Ⅰ组(P<0.01);试验Ⅰ组A/G极显著低于试验Ⅱ、Ⅲ组(P<0.01)。(3)血液免疫因子:试验Ⅱ组IL-1β、TNF-α浓度极显著高于对照组(P<0.01),显著高于试验Ⅰ组(P<0.05)。(4)血液激素:试验Ⅱ组PRL浓度极显著高于对照组(P<0.01),显著高于试验Ⅰ组(P<0.05)。【结论】补饲竹叶提取物能够提高泌乳期伊犁马的抗氧化性能。补饲竹叶提取物能够调节激素含量变化,改善泌乳期伊犁马乳汁分泌,提高其产奶性能。

Effects of Different Dosages of Bamboo Leaf Extract on Blood Biochemical Indexes of Yili Horse during Lactation

【Objective】 This experiment aims to investigate the effects of different doses of bamboo leaf extract on the antioxidant index, serum protein, immune index and hormone index of Yili horse during lactation in order to provide a theoretical basis for mastering the bamboo leaf extract that can be used as a new harmless additive to regulate the equine anti-oxidation, immune performance and hormones. 【Methods】 Twenty-four mares with similar body condition under grazing conditions on the same pasture in Zhaosu Stud-Farm of Yili Kazakh Autonomous Prefecture were selected as the research objects, and they were randomly divided into 4 groups with 6 horses in each group; The control group was not added with bamboo leaf extract, experiment I. Groups II and III were supplemented with bamboo leaf extract dosages 10 g/d, 20 g/d, and 30 g/d, respectively, and the feeding cycles were 60 days. On the 0 th, 15 th, 30 th, 45 th, and 60 th day, the mare's jugular vein blood was collected for analysis to study the effects of different supplementary feeding gradients on their blood biochemical indexes. 【Results】 (1) Antioxidant factors in blood: the concentration of MDA in test group III was significantly lower than that of control group and test group I (P<0.01). (2) Serum protein: The TP concentration of test group II was significantly lower than that of test groups I and III (P<0.05), and it was extremely significantly lower than that of control group (P<0.01); the concentration of ALB in test group I was significantly lower than that of control group (P<0.05), extremely significantly lower than test group III (P<0.01); test group II GLB concentration was extremely significantly lower than the control group, test group I (P<0.01); test group I A/G was extremely significantly lower than test II, Group III (P<0.01). (3) Blood immune factors: The concentration of IL-1β and TNF-α in the test group II was extremely significantly higher than that of the control group (P<0.01), and significantly higher than that in the test group I (P<0.05). (4) Blood hormones: The concentration of PRL in the test II group was extremely significantly higher than that in the control group (P<0.01), and significantly higher than that in the test I group (P<0.05). 【Conclusion】 Under the conditions of this experiment, supplementary feeding with bamboo leaf extract can improve the antioxidant performance of Yili horses during lactation and regulate changes in hormone content, improve milk secretion during lactation, and improve its milk production performance.