优异党参种质“凤党”离体繁殖技术

以“凤党”优株种子离体培养的无菌苗茎段为外植体,研究植物生长调节剂对其愈伤组织诱导及再分化的影响。结果表明,愈伤组织诱导的最适培养基为MS+0.5 mg·L^-1 NAA+1.0 mg·L^-1 6-BA,芽分化的最适培养基为MS+0.1 mg·L^-1 NAA+0.5 mg·L^-16-BA,生根的最适培养基为1/2 MS+0.2 mg·L^-1 NAA+0.2 mg·L^-1 IBA,愈伤组织诱导率、芽分化率及生根率分别可达91%、100%和97%。建立的离体再生植株快速繁殖体系,为“凤党”优异种质推广利用和工厂化育苗奠定了基础。

In vitro Propagation of An Excellent Germplasma of Codonopsis pilosula Fengdang

Codonopsis pilosula,a perennial species belongs to genus Codonopsis in family Campanulaceae,its root is a traditional tonic medicine used commonly in China.C.pilosula that is produced in Fengxian County of Shaanxi Province is named “Fengdang”,also famous for its rare and high quality.In this study,effects of different plant growth regulators on callus induction and redifferentiation were tested by using sterile stem explants in vitro that germinated from seeds of superior Fengdang plant.The results showed that the optimum medium for callus induction was MS with 0.5 mg·L^-1 NAA+1.0 mg·L^-1 6-BA.The optimal medium for bud differentiation was MS medium with 0.1 mg·L^-1 NAA+0.5 mg·L^-1 6-BA,while shoot rooting was best in the medium of 1/2 MS with 0.2 mg·L^-1 NAA+0.2 mg·L^-1 IBA medium.By using the media mentioned above,the rates of callus induction,bud differentiation and rooting could reach 91%,100% and 97%,respectively.The rapid propagation system of tissue culture established in this study laid foundation for high quality germplasm spread and utilization as well as industrial seedling of Fengdang.