假地豆响应缺磷胁迫的代谢衍生物分析

为探究假地豆(Desmodium heterocarpon)对缺磷胁迫的生理和代谢响应，本研究通过水培试验，分析了正常供磷(+P处理)和缺磷(-P处理)对假地豆生长及代谢物积累的影响。结果表明：缺磷胁迫降低了假地豆地上部干重和磷含量，但促进了根系生长。随后，代谢组共检测到假地豆叶片和根系的267个代谢物，可被分为12类。其中，叶片和根系的缺磷胁迫差异累积代谢物(DAMs)均为79个。缺磷处理导致假地豆叶片和根系的原花青素、槲皮素及其衍生物的积累显著增加，而2′-脱氧腺苷5′-单磷酸(dAMP)的积累显著减少。此外，缺磷处理使根系的8个有机酸和4个糖及醇类的积累显著增加。综上，假地豆通过促进根系生长和调整代谢物积累来提高对缺磷胁迫的适应性。本研究将为进一步解析假地豆适应缺磷胁迫的机理提供理论参考。

Metabolic Derivatives Analysis of Desmodium heterocarpon in Response to Phosphorus Deficiency Stress

To investigate the physiological and metabolic responses of Desmodium heterocarpon to phosphorus(P) deficiency stress,the effects of normal P supply (+P treatment) and P deficiency (-P treatment) on the growth and metabolite accumulation of D.heterocarpon were analyzed by hydroponic experiments. The results showed that P deficiency stress decreased shoot dry weight and P content of D.heterocarpon,but promoted root growth. Subsequently,the metabolome detected a total of 267 metabolites in the leaves and roots of D.heterocarpon,which could be classified into 12 categories. Among them,the differential accumulation metabolites (DAMs) of P deficiency stress in both leaves and roots were 79. The P deficiency treatment resulted in a significant increase in the accumulation of proanthocyanidins,quercetin,and their derivatives,and a significant decrease in the accumulation of 2'-deoxyadenosine 5'-monophosphate (dAMP) in the leaves and roots of D.heterocarpon. In addition,P deficiency treatment significantly increased the accumulation of 8 organic acids and 4 sugars and alcohols in the root system. In conclusion,D.heterocarpon improves its adaptation to P deficiency stress by promoting root growth and adjusting metabolite accumulation. This study will provide a theoretical reference for further analysis of the mechanism of adaptation to P deficiency stress in D.heterocarpon.