| 外壳蛋白基因片段介导的高抗TuMV研究 |
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| 引用本文： | 曾钢,叶艳英,曹鸣庆,马荣才,唐乐尘,姚磊.外壳蛋白基因片段介导的高抗TuMV研究[J].中国农业科技导报,2013,15(2):83-88. |
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| 作者姓名： | 曾钢 叶艳英 曹鸣庆 马荣才 唐乐尘 姚磊 |
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| 作者单位： | (1.北京市农林科学院农业生物技术研究中心, 北京 100097,2.福建农林大学, 福州 350002) |
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| 基金项目： | 国家863计划项目(2008AA10Z154);北京市自然科学基金(6092010)资助 |
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| 摘 要： | 为了获得对芜菁花叶病毒（TuMV）高度稳定的抗性，选取TuMV的外壳蛋白（CP）基因近3′端保守区共377 bp的核苷酸序列，构建了抗TuMV的发卡结构RNAi载体，并转化了TuMV的天然宿主拟南芥。转基因植株用北京地区TuMV主要流行的强致病株系BJ-C4人工接种。鉴定的8个转基因株系中有3个株系表现出对TuMV的高度抗性。定量PCR结果显示，高抗转基因株系体内几乎检测不到病毒的积累。
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| 关 键 词： | 芜菁花叶病毒 外壳蛋白基因 发卡结构 RNA干扰 抗性 |
Research of High Resistance to Turnip Mosaic Virus Mediated by Coat Protein Gene Segment |
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| ZENG Gang,YE Yan-ying,CAO Ming-qing,MA Rong-cai,TANG Le-chen,YAO Lei.Research of High Resistance to Turnip Mosaic Virus Mediated by Coat Protein Gene Segment[J].Journal of Agricultural Science and Technology,2013,15(2):83-88. |
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| Authors: | ZENG Gang YE Yan-ying CAO Ming-qing MA Rong-cai TANG Le-chen YAO Lei |
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| Institution: | (1.Beijing Ago-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097|2.Fujian Agriculture and Forestry University, Fuzhou 350002, China) |
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| Abstract: | In order to get stable high resistance to turnip mosaic virus (TuMV), a 377-nucleotide (nt) segment near 3′ conserved region of TuMV coat protein (CP) gene was chosen to construct hairpin RNAi vector, and transformed into Arabidopsis thaliana. The transgenic plants were inoculated with TuMV isolate BJ-C4, which was of high pathogenicity and mainly epidemic in Beijing area. Three of the 8 transgenic lines showed hyper-resistance to TuMV. Quantitative PCR results revealed that the accumulation of virus was hardly to detect in highly resistant transgenic plants. |
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| Keywords: | turnip mosaic virus coat protein gene hairpin structure RNAi resistance |
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