辣木组织培养试验研究

辣木种子消毒后,剥壳后接种在MS培养基上,10天能萌发,种子萌发率达79%。适合辣木种子的消毒方法是:双氧水浸种6～8 h,清水洗净,0.1%升汞消毒25～40 min;室温下,发芽种子的茎段在MS 6-BA 0.1mg/L KT0.5mg/L 蔗糖30% 琼脂6g/L的培养基上诱导产生不定芽,繁殖系数在6倍以上;不定芽切割后在MS 6-BA 0.6mg/L NAA 0.1mg/L 蔗糖30% 琼脂6g/L继代增殖,增殖倍数可达5.8倍;生根诱导在1/2 MS IBA 0.05～0.5 蔗糖15% 琼脂6g/L培养基上均能获得健壮的生根苗,生根率达80%以上,IBA 0.1mg/L时,达98%;移栽苗圃成活率可达80%。辣木各阶段最佳的PH值范围是5.8～6.2。

Study on Tissue Culture of Moringa Lam

The sterilized seeds of Moringa oleifera Lam started to germinate after they were striped and growned on MS media for 10 days.The germinate rate was up to 79%.The suitable disinfection way of M.oleifera's seeds was that soaked by H2O2 for 6~8h,cleaned by water,then sterilized with 0.1% HgCl2 25~40min.Under the common temperature,the sprig of germinated seeds could produce bud cluster and proliferation on the media of MS 6-BA 0.1 mg/L KT 0.5 mg/L cane suger 30% agar 6g/L,and the proliferation rate could be more than 6 times.The media for multiplication was MS 6-BA0.6mg/L NAA0.1mg/L cane suger30% agar 6 g/L,and the proliferation rate could be 5.8;The suitable media for rooting was 1/2 MS IBA 0.05~0.5 Suger 15%,and the rooting rate could be over 80%,the best was up to 98% when IBA was 0.1 mg/L.The survival ratio was 80% when grown in nursery.The best pH range is 5.8~6.2.