Infectious haematopoietic necrosis virus from salmonids cultured in Korea |
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| Authors: | M A PARK S G SOHN S D LEE S K CHUN J W PARK J L FRYER Y C HAH |
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| Institution: | Pathology Division, National Fisheries Research and Development Agency, Kyongnam, Korea;Department of Fish Pathology, National Fisheries University of Pusan, Pusan, Korea;Department of Microbiology, University of Ulsan, Kyongnam, Korea;Department of Microbiology, Oregon State University, Corvallis, Oregon, USA;Department of Microbiology, Seoul National University, Seoul, Korea |
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| Abstract: | Abstract. Four isolates of infectious haematopoietic necrosis virus (IHNV) were obtained from rainbow trout, Oncorhynchus mykiss (Walbaum), and masu salmon, Oncorhynchus masou (Walbaum), fry during epizootics at hatcheries in Korea. The four isolates of IHNV were compared with three from salmonids in the USA (SRCV, OSV and RB-76) by analysis of virion proteins in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and neutralization tests, with two monoclonal antibodies raised against SRCV (MAb SRCV/A4) and RB-76 (MAb RB/B5). Based on the antigenicity and the size of the structural proteins, one Korean isolate from masu salmon (SCS) is similar to RB-76 and is an electropherotype 1. The other three isolates from rainbow trout (PRT, YRT and MRT) were identical to each other in the mobilities of their virion proteins in SDS-PAGE, and although their nucleocapsid (N) proteins comigrated with that of the RB-76 isolate, they differed from RB-76 in the smaller matrix 2 (M2) protein they contained. In addition, the three Korean isolates (PRT, YRT and MRT) could be divided into two groups by reactivity with MAb RB/B5. While the YRT isolate was neutralized by this MAb, the PRT and MRT isolates were not, suggesting that there are at least two neutralizing antigenic variants of IHNV in Korea. |
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