| 小鼠胚胎干细胞培养相关影响因素的研究 |
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| 引用本文： | 蒙超衡,黄奔,石德顺,黎强,王殿君. 小鼠胚胎干细胞培养相关影响因素的研究[J]. 猪业科学, 2004, 21(9): 25-28 |
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| 作者姓名： | 蒙超衡 黄奔 石德顺 黎强 王殿君 |
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| 作者单位： | 广西大学动物繁殖研究所,广西,南宁,530005;广西中医学院,广西,南宁,530001;广西大学动物繁殖研究所,广西,南宁,530005;广西中医学院,广西,南宁,530001;广西大学辐射中心,广西,南宁,530005 |
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| 基金项目： | 国家863计划资助项目(2002AA206605) |
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| 摘 要： | 本实验主要探讨饲养层、白血病抑制因子(LIF)、水、血清对小鼠胚胎干细胞培养的影响。结果表明:饲养层细胞在小鼠胚胎干细胞的分化抑制过程中有一定作用,而起主导作用的是外源白血病抑制因子;;经亚沸处理的MilliQ水,能显著提高小鼠胚胎干细胞保持未分化状态的能力;;血清的品质对小鼠胚胎干细胞的培养效果影响显著(p<0.05)。
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| 关 键 词： | 胚胎干细胞 培养 饲养层 LIF 水 血清 |
| 文章编号： | 1008-9381(2004)09-0025-05 |
| 修稿时间： | 2004-08-11 |
Studies Related to Factors Affecting the Growth of Mouse Embryonic Stem Cells |
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| Meng Chao-heng, Huang ben Shi De-shun Li Qiang Wang Dian-jun. Studies Related to Factors Affecting the Growth of Mouse Embryonic Stem Cells[J]. Swine Industry Science, 2004, 21(9): 25-28 |
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| Authors: | Meng Chao-heng Huang ben Shi De-shun Li Qiang Wang Dian-jun |
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| Affiliation: | Meng Chao-heng1,2 Huang ben1 Shi De-shun1 Li Qiang2 Wang Dian-jun3 |
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| Abstract: | Factors affecting in vitro growth of mouse embryonic stem cells were i nvestigated in this study. Feeder layer has certain action in suppressing ES cel l differentiation in culture, while exogenous LIF play predominant role in main taining ES cells undifferentiation. Purification of Milli-Q water by sub-boiling distilled and employment of qualified FBS can improve the ability of ES cells t o maintan their undifferentaion state in vitro. |
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| Keywords: | ES culture feeder water serum LIF |
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