|
|||||
|
|
| 免疫抗体压力下PRRSVGD株Nsp2和GP5基因变异分析 | |
| 引用本文： | 牛晓芸,李康宁,贺东生.免疫抗体压力下PRRSVGD株Nsp2和GP5基因变异分析[J].广东畜牧兽医科技,2012,37(6):29-31,37. |
| 作者姓名： | 牛晓芸 李康宁 贺东生 |
| 作者单位： | 1. 华南农业大学兽医学院,广东广州,510642 2. 中牧实业股份有限公司,北京,100070 3. 华南农业大学兽医学院,广东广州510642 广东省兽用生物制品技术研究与应用企业重点实验室,广东广州510640 |
| 摘 要： | 以PRRSVGD-2007株为材料，在自然条件和抗体压力下连续传至40代后，分别命名为PRRSV—GD—f40和PRRSV—GDAb—f40，进行RT—PCR，扩增Nsp2基因部分序列和GP5基因全长，克隆并测序。应用序列分析软件将测序结果和已经发表的PRRSV毒株进行比对，结果显示：Nsp2基因扩增片段大小均为796bp，自然传代和抗体压力下传代后的新毒株和母源毒株的相似性分别为98．0％和97．9％；扩增的GP5基因大小为603bp，编码200个氨基酸，和母源毒株的相似性分别为99．2％和98．8％。 |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 Nsp2 GP5 序列分析 |
Variation Analysis of the Nsp2 and GP5 Gene ofPRRSV (GD strain) under Antibody Selective Pressures |
|
| Niu Xiaoyun,Li Kangning,He Dongsheng.Variation Analysis of the Nsp2 and GP5 Gene ofPRRSV (GD strain) under Antibody Selective Pressures[J].Guangdong Journal of Animal and Veterinary Science,2012,37(6):29-31,37. | |
| Authors: | Niu Xiaoyun Li Kangning He Dongsheng |
| Institution: | 1,2(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;2.Guangdong Veterinary Biological Products Technology Research and Application Enterprise key Laboratories,Guangzhou 510640,China;3.China Animal Husbandry Industry CO.,Ltd,Beijing,100070,China) |
| Abstract: | PRRSV strain GD was continuously propagated in Marc-145 cells for 40 passages without PRRSV antibody or with PRRSV antibody and the viruses after passages were named RRRSV-GD-f40 or RRRSV-GDAb-f40 respectively. Nsp2 and GP5 genes were cloned and sequenced. Comparison results showed that the amplification fragment of Nsp2 gene of all isolates were 796 bp long and shared 98.0% or 97.9% similirities with maternal strain respectively. The GP5 genome analyses showed that their cDNAs of GP5 were 603bp long and shared 99.2% or 98.8% similirities with maternal strain respectively. |
| Keywords: | PRRSV Nsp2 GP5 Sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |