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| Study on FSHR and LHR mRNA Levels of Different BMPRIB Genotypes of Small Tail Han Sheep During the Oestrum | |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | The relationship between different BMPRIB genotypes of Small Tail Han sheep and FSHR and LHR mRNA levels during the oestrum was studied using semiquantitative PCR. The results indicated that FSHR mRNA extracted from the right ovary of BB (1.14 ± 0.11) ewes showed higher levels compared with AA (0.44 ± 0.11) and AB (0.36±0.08) ewes (P ＜ 0.01), and LHR mRNA extracted from the right ovary of BB (0.42±0.02) ewes showed significantly higher levels compared with AA (0.23 ±0.02) and AB (0.25 ±0.04) ewes (P＜0.01); however, the mRNA extracted from the left ovary showed no significant difference in levels among the genotypes during the oestrum. It indicated that the fecundity induced by a mutation of BMPRIB in Small Tail Han sheep may be related to the changes of the mRNA expression of LHR and FSHR in ovary. |
| 关 键 词： | 小尾寒羊 发情期 信使核糖核酸 BMPRIB LHR FSHR |
Study on FSHR and LHR mRNA Levels of Different BMPRIB Genotypes of Small Tail Han Sheep During the Oestrum |
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| Authors: | JIA Cun-ling LI Ning WEI Ze-hui ZHU Xiao-ping LIU Hai-ying JIA Zhi-hai |
| Institution: | 1. College of Animal Science and Technology, China Agricultural University, Beijing 100094, P.R. China;College of Animal Science and Technology, Northwest A&F University, Yangling 712100, P.R. China 2. National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, P.R. China 3. College of Animal Science and Technology, China Agricultural University, Beijing 100094, P.R. China |
| Abstract: | The relationship between different BMPRIB genotypes of Small Tail Han sheep and FSHR and LHR mRNA levels during the oestrum was studied using semiquantitative PCR. The results indicated that FSHR mRNA extracted from the right ovary of BB (1.14±0.11) ewes showed higher levels compared with AA (0.44±0.11) and AB (0.36±0.08) ewes (P < 0.01), and LHR mRNA extracted from the right ovary of BB (0.42±0.02) ewes showed significantly higher levels compared with AA (0.23±0.02) and AB (0.25±0.04) ewes (P < 0.01); however, the mRNA extracted from the left ovary showed no significant difference in levels among the genotypes during the oestrum. It indicated that the fecundity induced by a mutation of BMPRIB in Small Tail Han sheep may be related to the changes of the mRNA expression of LHR and FSHR in ovary. |
| Keywords: | BMPRIB Small Tail Han sheep FSHR LHR fecundity |
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