| 杨树腐烂病生防放线菌筛选及其活性产物研究 |
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| 引用本文： | 原犇犇,曹支敏. 杨树腐烂病生防放线菌筛选及其活性产物研究[J]. 西北农林科技大学学报(自然科学版), 2007, 35(4): 139-144 |
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| 作者姓名： | 原犇犇 曹支敏 |
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| 作者单位： | 西北农林科技大学,林学院,陕西,杨凌,712100 |
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| 基金项目： | 全国高等学校优秀博士学位论文作者专项基金 |
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| 摘 要： | 通过培养基平板对峙培养,从292株放线菌中筛选出了8株对杨树腐烂病病原菌(Valsa sordida)有较强拮抗作用的菌株,再结合发酵液的生长抑制率测定,筛选出了1株抑菌效果最好的菌株,对其发酵液的萃取液进行了薄层层析分析,初步确定抑菌活性是由一种极性较弱的物质引起的,并对该物质的理化性质进行了研究,结果表明,此活性物质无挥发性,对酸、温度和紫外线都较稳定。
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| 关 键 词： | 放线菌 杨树腐烂病 薄层层析 活性产物 理化性质 |
| 文章编号： | 1671-9387(2007)04-0139-06 |
| 收稿时间： | 2006-10-19 |
| 修稿时间： | 2006-10-19 |
Screening of biocontrol actinomycetes of Valsa sordida and study on bioactive metabolite |
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| YUAN Ben-ben,CAO Zhi-min. Screening of biocontrol actinomycetes of Valsa sordida and study on bioactive metabolite[J]. Journal of Northwest A&F University（Natural Science Edition）, 2007, 35(4): 139-144 |
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| Authors: | YUAN Ben-ben CAO Zhi-min |
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| Affiliation: | (College of Forestry,Nothewest A & F University ,Yangling ,Shaanxi 712100,China) |
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| Abstract: | Through agar plate antagonism,8 antagonistic actinomycetes which had strong antagonism to Valsa sordida were screened. Through bioactive test of ferment filtrate, one strain which had best bioac-tivity was screened from the 8 strains,and the TLC result of the extract from its ferment fluid revealed that the bioactivity was caused by one weaker polarity substance. Then the characteristics of this bioactive substance were studied. The results showed that this bioactive metabolite did not have volatility,and it was acid and heat and UV stable. |
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| Keywords: | actinomycetes Valsa sordida TLC bioactive metabolite characteristics |
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