| 勿忘我组织培养技术研究 |
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| 引用本文： | 陆玲丽,李枝林,姜勤,阮文进. 勿忘我组织培养技术研究[J]. 江西农业学报, 2009, 21(6): 40-42,45 |
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| 作者姓名： | 陆玲丽 李枝林 姜勤 阮文进 |
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| 作者单位： | 云南农业大学,园林园艺学院,云南,昆明,650201;云南省玉溪市通海县经济作物工作站,云南,玉溪,652700 |
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| 摘 要： | 采用勿忘我蓝A和蓝B作为材料来源,通过外植体分化出不定芽。在相同起始培养基中蓝A产生不定芽效果比蓝B好。再以MS和1/2MS为基本培养基,进行增殖培养和生根培养的研究。试验结果表明:蓝A和蓝B均在MS+6-BA0.4 mg/L+NAA 0.1 mg/L上增殖倍数最高,蓝A在MS+6-BA 0.1 mg/L+NAA 0.1 mg/L上小苗叶色绿、长势良好,蓝B在MS+6-BA 0.2 mg/L+NAA 0.1 mg/L上小苗叶色浓绿、长势良好;蓝A和蓝B生根的最适配方均为1/2MS+NAA 0.3 mg/L;勿忘我小苗蓝A和蓝B都是在腐殖土∶珍珠岩∶木屑=2∶2∶1的介质上移栽成活率高。
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| 关 键 词： | 勿忘我 外植体 增殖培养 生根培养 移栽 |
Study on Tissue Culture of Limonium sinuatum |
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| LU Ling-li,LI Zhi-lin,JIANG Qin,RUAN Wen-jin. Study on Tissue Culture of Limonium sinuatum[J]. Acta Agriculturae Jiangxi, 2009, 21(6): 40-42,45 |
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| Authors: | LU Ling-li LI Zhi-lin JIANG Qin RUAN Wen-jin |
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| Affiliation: | 1.Faculty of Horticulture and Landscape;Yunnan Agricultural University;Kunming 650201;China;2.Economic Crop Workstation of Tonghai County in Yunnan Province;Yuxi 652700;China |
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| Abstract: | In this experiment,the tissue culture techniques of Limonium sinuatum were studied.The results indicated that Limonium sinuatum-A generated adventitious buds better than Limonium sinuatum-B on the same basic medium.On MS+6-BA 0.4 mg/L+NAA 0.1 mg/L,both Limonium sinuatum-A and Limonium sinuatum-B had the highest proliferation times,the plantlets of Limonium sinuatum-A grew well and their color was green on MS+6-BA 0.1 mg/L+NAA 0.1 mg/L,and the plantlets of Limonium sinuatum-B grew well and their color was th... |
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| Keywords: | Limonium sinuatum Explant Proliferation Rootage Transplanting |
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