| 花生高质量DNA提取方法研究 |
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| 引用本文： | 张宁洁. 花生高质量DNA提取方法研究[J]. 安徽农学通报, 2007, 13(8): 101-102. DOI: 10.3969/j.issn.1007-7731.2007.08.048 |
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| 作者姓名： | 张宁洁 |
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| 作者单位： | 广西大学,广西南宁,530003;广西农科院重点实验室,广西南宁,530003 |
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| 基金项目： | 广西自然科学基金项目(桂科回0342003) |
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| 摘 要： | 本文采用改良CTAB法提取花生的DNA,在药品浓度和时间掌握上有所不同,却得到意想不到的结果,提取出的DNA样品产率在57.07-87.27ng/ml.(稀释10倍) ,DNA 质量和纯度较高,260nm/ 280nm 光密度比值在1.8-2.0 之间.所提取的DNA可直接用于限制性内切酶酶切、连接,也可用于随机引物PCR 扩增.
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| 关 键 词： | 改良CTAB法 DNA 提取 浓度 纯度 |
| 文章编号： | 1007-7731（2007）08-101-02 |
| 收稿时间： | 2007-04-09 |
| 修稿时间： | 2007-04-09 |
Extracting High Quality DNA of Peanut |
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| Zhang Ning-jie. Extracting High Quality DNA of Peanut[J]. Auhui Agricultural Science Bulletin, 2007, 13(8): 101-102. DOI: 10.3969/j.issn.1007-7731.2007.08.048 |
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| Authors: | Zhang Ning-jie |
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| Affiliation: | 1 Agriculture College of Guangxi University, Nanning, 530003 ; 2 Key Laboratory of Guangxi Academy of Agricultural Science , Nanning, 530003 |
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| Abstract: | We extracted DNA of peanut by improved CTAB way.Based on the different medical density and time control,we receive perfect result.The DNA productivity is between 57.07ng/mL and 87.27ng/mL.The quality and purity of DNA are high.Photic density of 260ng/280ng is between 1.8 and 2.0.The extracted DNA can be directly used for the sever of restriction enzyme and its ligation or the PCR amplification by random primer. |
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| Keywords: | improved CTAB way DNA extract density purity |
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