用农杆菌介导法将大豆球蛋白基因导入水稻

在克隆水稻谷蛋白基因Gt1启动子的基础上,以农杆菌双元载体pCAMBIA1301为起点,构建出以该启动子带动大豆球蛋白A1aB1b亚基基因表达的双元载体,并用农杆菌介导法转化水稻获得转基因植株.经PCR检测和后代分析,大豆球蛋白基因已整合入水稻基因组,并在后代中稳定遗传.

Introduction of soybean glycinin gene into rice (Oryza sativa L. ) with Agrobacterium-mediated trans-formation.

Based on the previous work of cloning of upstream sequence of a rice glutelin gene Gt1, an Agrobacterium binary vector based on pCAMBIA1301 was reconstructed with a soybean glycinin gene directed by the tissue specific promoter, and was used in rice transfomation. The target gene was successfully introduced into calli of an early japonica rice variety with Agrobacterium mediated transformation and several transgenic plants were obtained. PCR analysis of the transformed plants and Hyg resistance tests of T 0 seeds showed the integration of the target gene and its stable inheritance to offsprings.