Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction |
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Authors: | Pinches Mark D G Helps Christopher R Gruffydd-Jones Tim J Egan Kathy Jarrett Oswald Tasker Séverine |
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Affiliation: | University of Bristol, School of Clinical Veterinary Science, Langford House, Langford, Bristol BS40 5DU, UK. m.pinches@bristol.ac.uk |
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Abstract: | In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods. |
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