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| 海洋侧孢短芽孢杆菌S-12-86溶菌酶的分离纯化与冻干技术研究 | |
| 引用本文： | 纪存朋,孙谧,于建生,郑媛. 海洋侧孢短芽孢杆菌S-12-86溶菌酶的分离纯化与冻干技术研究[J]. 渔业科学进展, 2010, 31(1): 104-109 |
| 作者姓名： | 纪存朋 孙谧 于建生 郑媛 |
| 作者单位： | 1. 中国水产科学研究院黄海水产研究所,青岛,266071;青岛科技大学化工学院,266042 2. 中国水产科学研究院黄海水产研究所,青岛,266071 3. 青岛科技大学化工学院,266042 |
| 基金项目： | 国家863计划项目(2006AA10Z349和2007AA091602)资助 |
| 摘 要： | 海洋微生物溶菌酶发酵液经低温离心、超滤浓缩、乙醇提取、Superdex 75 10/300凝胶层析和反相高效液相色谱层析纯化得到电泳纯海洋溶菌酶,分子量为17.1 kD,比活达到3 987.7 U/mg,纯度提高41.98倍,活力回收率为21.7%。对该酶冷冻干燥过程的研究结果表明,海藻糖、蔗糖和麦芽糖对该酶均有一定的冻干保护作用。其中,以海藻糖的保护效果最佳。0.5 mol/L海藻糖与20 mg/ml吐温80复合作为冻干保护剂,使冻干后的溶菌酶活性维持在95%以上,为该酶进一步研究和应用提供了稳定的酶制剂。 |
| 关 键 词： | 海洋微生物 溶菌酶 分离纯化 冷冻干燥 |
| 收稿时间： | 2009-03-05 |
| 修稿时间： | 2009-04-30 |
Purification and lyophillization of Lysozyme produced by marine microorganism |
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| JI Cun-peng, SUN Mi YU Jian-sheng ZHENG Yuan. Purification and lyophillization of Lysozyme produced by marine microorganism[J]. Progress in Fishery Sciences, 2010, 31(1): 104-109 | |
| Authors: | JI Cun-peng SUN Mi YU Jian-sheng ZHENG Yuan |
| Affiliation: | 1 Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2 College of Chemical Engineering;Qingdao University of Science & Technology;266042 |
| Abstract: | Cell-free supernatant with marine microorganism lysozyme was prepared by centrifugation of culture broth,ultrafiltration and concentration.The crude lysozyme was purified 41.98 fold to electrophoretic homogeneity with recovery of 21.7% and specific activity of 3 987.7 U/mg by extracting with ethanol,Superdex 75 10/300 chromatography and reversed-phase HPLC.The relative molecular weight of this lysozyme was 17.1 kD determined by SDS-PAGE electrophoresis.Preparations of lysozyme from native marine microorgani... |
| Keywords: | Marine microorganism Lysozyme Purification Lyophillization |
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