2种升温方式对毛蚶呼吸代谢及免疫相关酶活性的影响

采用室内控温试验,设置15、19、23、27℃4个温度梯度,每个温度梯度分别设置急剧升温和缓慢升温2种方式,研究2种升温方式对毛蚶呼吸代谢及免疫相关酶活性的影响.试验结果显示,在试验温度范围内,2种升温方式下毛蚶的耗氧率和排氨率均随海水温度的升高先增后降,于23℃达到最大值.2种升温方式下毛蚶耗氧率Q10值和排氨率Q1...

Effects of Two Warming Patterns on Respiratory Metabolism and Immune-related Enzyme Activities of Ark Shell Scapharca subcrenata

The effects of changes in temperature on physiological and biochemical activities of ark shell Scapharca subcrenata were studied in a laboratory.Ark shell was held at sea water temperature of 15℃for 2 weeks and then divided into two groups:in one group the ark shell was directly moved into a tank at 19℃,23℃and 27℃,and in the other group the ark shell was slowly exposed to the same temperature at a warming rate of 1℃/12 h.It was found that the oxygen consumption rates and ammonia excretion rates of ark shell were increased first and then decreased with the increase in seawater temperature within the experimental temperature,with the maximal values at 23℃.The ark shell had the maximal oxygen consumption Q10 and ammonia excretion Q10 at 19—23℃,and had the minimal O∶N ratio at 19℃,under the rapid and slow warming patterns.Under the rapid warming pattern,activities of catalase(CAT),superoxide dismutase(SOD),acid phosphatase(ACP),and lysozyme(LSZ)were increased at first and decreased afterwards in ark shell exposed to high temperature(23℃or 27℃)for 96 h and reached the maximum at 6 h(P<0.05).There was more obvious effect on physiological and biochemical activities of ark shell under sudden warming than under slow warming.The findings will help to clarify the mechanism of temperature changes on physiological and biochemical activities of ark shell and provide reference data for the cultivation and production of ark shell.