仿刺参SARM基因的克隆、表达和功能研究

采用RACE方法克隆了仿刺参TLR信号通路MyD88非依赖途径接头分子SARM,命名为AjSARM.AjSARM基因的序列全长3874 bp,开放阅读框为2412 bp,编码803个氨基酸.通过SMART软件对Aj SARM蛋白进行蛋白结构预测,发现Aj SARM蛋白由2个SAM结构域和1个C端TIR结构域组成.采用实...

Characterization,Expression and Function of SARM in Sea Cucumber Apostichopus japonicus

SARM,an adaptor of MyD88 independent signal pathway,was cloned in sea cucumber Apostichopus japonicus,named AjSARM by RACE method.The full length of AjSARM was 3874 bp with an ORF of 2412 bp encoding 803 amino acids.Structure prediction by SMART indicated that AjSARM contained two sterile motifs(SAM)and a C-terminal TIR domain.Quantitative real-time PCR was selected to examine AjSARM expression at different developmental stages of sea cucumber.AjSARM was rapidly up-regulated from auricularia at nervous development stage.It was indicated that AjSARM played an important role in the process of development.After four pathogens stimulus,the expression of AjSARM was inhibited at 4 h and 24 h in the groups of Vibrio splendidus,Pseudoalteromonas nigrifacien and Shewanella baltica chanlenged,but upregulated at 24 h in Bacillus cereus group.Except in S.baltica group,AjSARM reached top expression level all in other three groups at 96 h.The dynamic expression patterns of AjSARM at different times in response to different pathogens proved that it participated in the immune reaction in sea cucumber coelomocytes.The structure and expression analyses of AjSARM help to explore its functions as an adaptor and will lay foundations for the evolution study of immune system in echinoderm and disease prevention in sea cucumber.